resulting in the formation of a hydroperoxide and eventually a ketone (after removal of water). Since the goal was to have a mixture of 50% fluorene conversion‚ Thin Layer Chromatography (TLC) was carried out several times to assess the rate of the reaction. In order to separate the aqueous composites from the solution
Premium Chemistry Hydrogen Chemical reaction
on a different piece of paper. Then the spot was allowed to dry and sprayed with ninhydrin to test for the presence of amino acid with gentle heat applied from hot plate. TLC of Amino Acid Fractions: Another TLC plate was spotted comparing the most concentrated fraction from each of the pH levels with the unknow mixture. The TLC developing jar was prepared with 4:1:1 propanol/acetic acid/water. The plate was placed in developing jar once spotted and allowed to develop until the solvent front reached
Premium Amino acid Chromatography Acid
reaction will be followed by TLC as directed in the Reaction Conditions section. Glass backed TLC plates will be created with the following three spots: Ketone Standard/Co-Spot/Crude Reaction Mixture. Prepare the TLC plate while the reaction is being set up. To the initial solution of 4-tert-butylcyclohexane in methanol‚ before adding the reducing solution‚ insert a TLC capillary. Use this sample as your ketone standard. Do NOT stop the reaction before checking the progress by TLC. If the reaction is not
Premium Hydrogen Alcohol Chemical reaction
cause the ferrocene to add either one acetyl group to an aromatic ring or add two acetyl groups to each of the aromatic rings. In order to determine how well this process had worked we employed: IR spectra analysis‚ column chromatography‚ and a little TLC. This experiment is relevant in today’s highly industrialized world. By utilizing many of the techniques we employ in this lab‚ a company can synthesize new types of materials or composites that could revolutionize an industry. Background When
Premium Oxygen Chemistry Hydrogen
the gums for the flavor. The reaction that takes place in this experiment is a esterification. In this experiment‚ the carboxyl group of the acetic acid joins with the hydroxyl group of the isopently alcohol‚ catalyzed by the presence of sulfuric acid‚ through a dehydration reaction‚ in which water is removed. This allows for an ester bond to form in the second carbon of the isopentyl acetate (banana oil). Esterification of the acetic acid with isopentyl alcohol is the reaction‚ which prepared the
Premium Ester Alcohol Acetic acid
Chemical Separation Introduction: The objective of this experiment was to extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon
Premium Thin layer chromatography Purple Color
Experiment 3 Preparation of 2‚ 4-Dinitrodiphenylamine Courtney Elahee Claim: The evidence will show that the red solid collected after recrystallization from the nucleophilic aromatic substitution of 2‚ 4-dinitrobromobenzene and aniline has the characteristics of 2‚ 4-dinitrodiphenylamine. Evidence: The procedure was carried out as described by Ault1 and the lab manual2. 2‚4-dinitrobromobenzene (0.92g‚ 3.75mmol‚ pale yellow solid) and aniline (0.7mL‚ 0.7g‚ 7.5 mmol) were boiled in ethanol (10mL)
Premium Thin layer chromatography Chromatography
B: Syn dihydroxylation and visualization using KMnO4 (on a TLC plate) |Important steps |Observations | |TLC after 15 min‚ dipped in KMnO4 solution |All spots turned yellow-brown
Premium Oxygen Hydrogen Hydrogen peroxide
Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined
Premium Solvent Chromatography Thin layer chromatography
chromatography (TLC) and measure the Rf values of the colored components obtained herein. For column chromatography‚ the sample prepared was loaded into a Pasteur pipette plugged with cotton and uniformly packed with silica gel. The eluents used were 7 mL hexane:acetone (7:3)‚ 5 mL hexane:acetone (1:1)‚ 5 mL acetone‚ and 5 mL acetone:MeOH (1:1); the collected eluates were dark green‚ light green‚ yellow green‚ and yellow. For thin layer chromatography‚ the eluates were applied in spots on a TLC plate pre-coated
Premium Thin layer chromatography Chromatography