2-15-11 Purpose: The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part
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Student Name: Melissa Tatum Student ID: 4593119 Date: 7 Dec 2014 Course and Section Number: SCIN131 A004 Fall 14 Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds PART 1 Begin by viewing the following Thinkwell video 15.1.3 CIA Demonstration: Chromatography After you watch the above video‚ answer the questions below in sufficient detail: (a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned‚ and describe their differences in as much detail
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2) Improvisation Music Therapy literature documents the development and value of improvisation in clinical work working with different kind of clinical populations. Improvisation is a category of Music Therapy often used by therapists Within improvisation‚ there are many forms‚ methods and techniques. Therapists use improvisation in order to allow the clients to improve self-expression‚ creativity and communication using voice‚ movement and percussion instruments. The client makes up his or her
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To increase the percentage yield of ethyl ethanoate can be achieved by removing water or ester during esterification. Esterification is reversible reaction. For all reversible reactions‚ they have equilibrium constant‚ denoted by Kc. It should be the same if the reaction performed under same condition. In order to keep the Kc constant‚ the reaction will shift right
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perform a double elimination reaction by product gained to synthesize an alkyne‚ that is‚ 1‚2-diphenylacetylene. The two major techniques used in this lab were TLC analysis and UV-vis spectroscopy. TLC technique is non-destructive‚ that is‚ the molecules in the mixtures are separated physically without being chemically altered. Analytical TLC is used in drug analysis‚ consumer product monitoring‚ environmental pollution! studies‚ forensics and many other applications. Further‚ UV-vis method is based
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separation as TLC‚ but its advantage over TLC is the fact that large amounts of mixtures (over 5 g) can easily be treated by this method. There are‚ however‚ a number of important similarities between TLC and column chromatography which‚ when the two methods are used in complementary fashion‚ allow preparative separations to be accomplished easily and quickly. The same comments that were made concerning adsorbents and solvents for TLC apply also to column chromatography. In particular‚ by using TLC initially
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Unknown #1‚ caffeine‚ aspirin‚ and acetaminophen. Thin-Layer Chromatography is a technique for identifying substances using a mobile phase (developing solvent) that draws up through a stationary phase (TLC plate) and marks spots of differing distances for differing substances. (1) The TLC plate used was a silica gel stationary phase with plastic backing. It is very important to carefully handle the plate as it is very delicate and prone to flaking off‚ giving inaccurate readings. It is a
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Nitration of Acetanilide Introduction When organic compounds are nitrated it changes the composition and reaction of the compounds.[4] We could benefit from these changes‚ nitration of acetanilide produces a product called p- Nirtoanoline. P-Nitroaniline is commonly used as an intermediate in the synthesis of dyes‚ antioxidants‚ pharmaceuticals and gasoline. P-Nitroaniline is also used in gum inhibitors‚ poultry medicines and as a corrosion inhibitor. [2] Nitration is a type of chemical
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spot known compounds onto the same plate as your unknown compound and run the compounds side-by-side to compare. • The spots for the same compounds will not only have the same Rf value but also have the same appearance. Same Compound or Not? TLC can also be used to check the purity of a compound. A pure compound will always appear as a single spot (because it is a single compound) regardless of what solvent system is used for developing the plate. The solvent system should be neither very
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of the insoluble characteristics. After one week‚ the weights of the dried liquid samples on the watch glasses were taken. Four TLC plates were obtained and a line‚ about 1 cm from the bottom‚ was drawn across the plate. Each solid on the different watch glasses were scraped to obtain a small sample and placed into test tubes to be dissolved in acetone once again. A TLC chamber was then assembled by using a beaker slightly filled with solvent‚ 3:6:1 Toluene: EF2O: MeOH. Plates were then spotted along
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