Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical analysis ..................... 1 1. Flame Photometry ................................................................... 1 2. Fluorimetry ........................................................................... 10 3. Nephelometry .............................................................
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A diode is a two-terminal device‚ having two active electrodes‚ between which it allows the transfer of current in one direction only. Diodes are known for their unidirectional current property‚ wherein‚ the electric current is allowed to flow in one direction. Basically‚ diodes are used for the purpose of rectifying waveforms‚ and can be used within power supplies or within radio detectors. They can also be used in circuits where ’one way’ effect of diode is required. Most diodes are made from semiconductors
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ELECTRIC POWER UTILISATION AND ENERGY AUDITING UNIT – I ILLUMINATION‚ HEATING AND WELDING Nature-of radiation –definition – laws photometry – polar curves – lighting calculationsdesign of illumination systems (for residential‚ industrial‚ commercial and street lightings) - types of lamps-energy efficiency lamps. Methods of heating requirement of heating material-design of heating element-furnaces – Welding generator-welding transformer and its characteristics UNIT - II ELECTRIC TRACTION
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List of Chemistry Terms used in daily life α (alpha)‚ β (beta) α- (β-‚ γ-) ray spectrometer α-addition (alpha-addition) α-cleavage (alpha-cleavage) α-decay (alpha-decay) α-effect α-elimination α-expulsion in photochemistry α-oxo carbenes α-particle (alpha-particle) ’A’ value -factorquantity ab initio quantum mechanical methods abatement in atmospheric chemistry abeo- abiological abiotic abiotic transformation absolute activation analysis absolute activity‚ quantity absolute
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reproductive processes as well as decrease overall colony size. In our experiment‚ we plan to note the effects of salt on S. cerevisiae by both spot plating to note overall cell colony size and the number of total cells and through the use of electro photometry to also count total cell concentration. Results: In our experiment‚ we determined how the concentration of sodium chloride effected cell growth and proliferation. We allowed S. cerevisiae cells to grow in mediums of varying sodium chloride concentrations
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colorimeter. In colorimetric measurements‚ color of solution is compared with color of standard solutions in different concentrations. Measuring amount of substance in solution by helping intensity of transmittance light from solution is called photometry. Devices that used for this method is called photometer. This kind of devices have filter to adjust wavelength of light. If the device has a prism to do this‚ it is called spectrophotometer. In photometric measurements‚ concentration of colorless
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Reflection is the change in direction of a wavefront at an interface between two different media so that the wavefront returns into the medium from which it originated. Common examples include the reflection of light‚ sound and water waves. The law of reflection says that for specular reflection the angle at which the wave is incident on the surface equals the angle at which it is reflected. Mirrors exhibit specular reflection. In acoustics‚ reflection causes echoes and is used in sonar. In geology
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International Journal of Chemical and Analytical Science ISSN: 0976-1206 Research Article www.ijcas.info Physico-Chemical Characterization of Rubber Seed Oil (Hevea Brasiliensis) - A Promising Feedstock for Biodiesel Production VN Meena Devi*1‚ P Nagendra Prasad2‚ L Arul Mary Syndia2 ‚ M Rajakohila1 ‚ and VN Ariharan2 *1Department of Physics‚ Noorul Islam Centre for Higher Education‚ Noorul Islam University‚ Kumaracoil‚ Thuckalay – 629 180‚ Tamilnadu‚ India 2Department of Biotechnology‚ Sri Paramakalyani
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certain chemical solution can be identified is through a simple test called a flame test. A flame test works by placing a certain chemical solution in an open flame and noting the color the flame changes to. The color of the flame changes due to the fact that electrons are constantly moving between energy levels as stated in the Hutchinson Encyclopedia. The chemical involved in case study B will be identified using a flame test and extensive research
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