Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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Candy Chromatography: What makes those colors? The point of doing this project is to use paper chromatography to see which dyes are used in the coatings of your favorite colored candies. Chromatography is a technique for separating mixtures. Chromatography can be used to separate specific components from a complex mixture‚ based on molecular size or other chemical properties. It can also be used to identify chemicals‚ for example crime scene samples like blood‚ drugs‚ or explosive residue. Highly
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Chromatography Experiment of the Drosophila Eye Pigmentation Pathway Introduction: How can the use of chromatography help explain single gene mutations of the drosophila melanogaster bio-synthetic pigment pathway? This experiment was meant to help connect the mode of inheritance with different eye color mutations and pinpoint where they occur in the pigment pathway. The drosophila eye color is a result of two bio-synthetic eye color pathways‚ this is expressed as a trait or phenotype‚ a multigene
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Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens‚ using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water‚ D. Sellers used
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Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the column is referred
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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Instructor: Ma ’am Lioanag Section: Civil Engineering 1-19 Date Performed: July 27 ‚ 2013 Group No.: 3 Date Submitted: August 3 ‚ 2013 Experiment No. 4 : PAPER CHROMATOGRAPHY INTRODUCTION The objective of this experiment was to resolve a given sample into its components by paper chromatography‚ to evaluate the retention factor of each component in the sample and to compare the relative solubility of the various components of a mixture of colors in a given solvent. METHODOLOGY
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understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each
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experiment is to identify an unknown proprietary drug using thin-layer chromatography. The unknown’s behavior in thin-layer chromatography will be compared with that of its possible component analgesics. The possible unknowns and their analgesic ingredients will be Anacin (aspirin‚ caffeine)‚ Excedrin (acetaminophen‚ caffeine‚ aspirin)‚ Motrin (ibuprofen)‚ and Tylenol (acetaminophen). Introduction Thin-layer Chromatography(TLC) was the method used to figure out and identify the drug. TLC is done
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