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    SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification

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    favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This

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    Paper Chromatography Lab

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    Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly

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    Distillation Column Design

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    EXECUTIVE SUMMARY: The main objective of this project was to carry out a design on the Propane distillation column for a process that produces 1‚3 Butadiene. The raw materials used are Butane‚ Oxygen and Water and the process capacity is 100 000 tonnes per annum nominal. In the previous work it was stated that the plant is located in China. The main location of the plant is in the capital city of the Gansu Provence‚ Lanzhou. This was decided as the location over other provinces due to the excellent

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    Gas Chromatography Lab

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    Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method

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    Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or

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    Gas Chromatography Essay

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    Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because

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    Chromatography White Wine

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    Acids  in  White  Wine  Using  High Performance Liquid Chromatography    Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M.    Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City  Performed: December 9‚ 2011  Submitted: December 15‚ 2011        RESULTS AND DISCUSSION  solutions  containing  increasing  amounts  of  the  organic  acid.  These  solutions  were  contained  in  flasks  numbered  5‚  6‚  7‚  8‚  and  9.  (Refer  to  the  Appendix  to  see  components  of 

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    Trajan's Column Analysis

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    Backgrounds of monuments in order of year built‚ Trajan’s Column was designed by the architect Apollodorus of Damascus and commissioned by the Roman Senate in 107‚ it took six years to be built‚ therefore‚ the column was completed in 113 DA. The column measures 126 feet high and it used to end with a bronze statue of Trajan. The carvings on the tower represent different scenes of the narrative of the Dacian campaigns and wars‚ depicting different experiences his soldiers went through in different

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    Winogradsky column description Introduction The experiment of Winogradsky column is to observe microbiology interaction of microbes using pond mud. The aim of this paper is to describe structural and chemical features in Winogradsky column and to show the acknowledgement of microbial cells in Winogradsky column. Materials of experiment Pond mud Calcium sulfate Calcium carbonate Shredded paper Pond water Cylinder Aluminum foil Methods 1. Prepare pond mud 2. Add calcium sulfate

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