SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
Premium Chromatography High performance liquid chromatography Protein
favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
Premium Dye Chromatography Food coloring
Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
Premium Chemistry Solubility Solvent
EXECUTIVE SUMMARY: The main objective of this project was to carry out a design on the Propane distillation column for a process that produces 1‚3 Butadiene. The raw materials used are Butane‚ Oxygen and Water and the process capacity is 100 000 tonnes per annum nominal. In the previous work it was stated that the plant is located in China. The main location of the plant is in the capital city of the Gansu Provence‚ Lanzhou. This was decided as the location over other provinces due to the excellent
Premium Distillation Chemical engineering
Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
Premium Distillation Evaporation Water
Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
Premium Chromatography Solubility Gas
Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
Premium Temperature Thermodynamics Gas
Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to the Appendix to see components of
Premium Chemistry Acetic acid Oxygen
Backgrounds of monuments in order of year built‚ Trajan’s Column was designed by the architect Apollodorus of Damascus and commissioned by the Roman Senate in 107‚ it took six years to be built‚ therefore‚ the column was completed in 113 DA. The column measures 126 feet high and it used to end with a bronze statue of Trajan. The carvings on the tower represent different scenes of the narrative of the Dacian campaigns and wars‚ depicting different experiences his soldiers went through in different
Premium Texas
Winogradsky column description Introduction The experiment of Winogradsky column is to observe microbiology interaction of microbes using pond mud. The aim of this paper is to describe structural and chemical features in Winogradsky column and to show the acknowledgement of microbial cells in Winogradsky column. Materials of experiment Pond mud Calcium sulfate Calcium carbonate Shredded paper Pond water Cylinder Aluminum foil Methods 1. Prepare pond mud 2. Add calcium sulfate
Premium Photosynthesis Oxygen Bacteria