for H. sp. KHS3. To our knowledge‚ most of the studies on Halomonas sp. focused on degradation of monoaromatic rather than polycyclic and heterocyclic compounds. We have demonstrated that H. sp. KHS3 has the ability to growth using phenanthrene‚ fluorene and naphthalene as the only carbon source. Similar ability has been demonstrated by
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and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and 3222 cm-1 and the C=O stretch was found at 1685 cm-1. The IR for 9-fluorenone did not show any significant bonds except for at 1681 cm-1‚ the C=O stretch. To separate 4-aminobenzoate into an aqueous layer‚ HCl was added to protonate the NH2 side group and form a salt‚ creating its high solubility in water and low solubility
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(separately!) combine your three samples of benzocaine‚ and combine your three samples of fluorenone. (If you misread this and mix everything together‚ no worries‚ you can just repeat the extraction to separate them again.) Now‚ one group is going to recrystallize the benzoic acid from water‚ one group is going to recrystallize the benzocaine from ethanol and water‚ and one group is going to recrystallize the fluorenone from
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Extraction Report For experience 4c we were supposed to determine which one is the organic layer in three different tubes that has two layers. To determine which is aqueous (water) or organic‚ I used the technique of adding droplets of water to each tube. If the layer is water‚ then the drops of added water will dissolve in the aqueous layer and increase it’s volume. If the added water form droplets or a new layer‚ then it is the organic layer. Tube 1 (water and n-butyl chloride)‚ after adding
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Results and Discussion – Expt. 3 (Crystallization) Name: Parts A and B: 1. Please comment on the relative purity of your recrystallized salicylic acid (Hint: You should use your empirical data to support your claim). -The Salicylic acid that I obtained had a melting point range around 157-158 degrees Celsius for Part A. For Part B the melting point range was 156-157.5 degree Celsius. Part B‚ I would say the sample is less pure because of the notion that impurities lower the melting point
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while Succinimide looked like crystals. The two mixed melting point range trials confirmed this. Unknown number 18 is Benzoic Acid. A possible alternative would be Succinimide‚ because of the close melting points. Another possible alternative is Fluorene‚ which melts at slightly lower temperatures than my unknown does. Benzoic Acid is C7H6O2. Its structure is: My percent recovery was 28.26%. During the dissolving of my unknown‚ there was still undissolved solid. This could have led to a smaller
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Lab 3: Solubility of Organic Compounds Objectives: Understanding the relative solubility of organic compounds in various solvents. Exploration of the effect of polar groups on a nonpolar hydrocarbon skeleton. Introduction: The solubility of a solute (a dissolved substance) in a solvent (the dissolving medium) is the most important chemical principle underlying three major techniques you will study in the organic chemistry laboratory: crystallization‚ extraction‚ and chromatography.
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heating it. • Always wear department approved eye goggles at all times. • Long pants and closed toe shoes are required. Table of Reagents Reagent Mol. Wt. (g/mol) Bp ( ͦ C) Mp ( ͦ C) Density (g/ml) Acetanilide 135.17 304 113-115 1.219 Fluorene
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PAHs (Acenaphthene‚ Acenaphthylene‚ Anthracene‚ Benz[a]anthracene‚ Benzo[b]fluoranthene‚ Benzo[k]fluoranthene‚ Benzo[ghi]perylene‚ Benzo[a]pyrene‚ Chrysene‚ Dibenz[a‚h]anthracene‚ Dibenzo[a‚e]pyrene‚ 7‚12-Dimethylbenz[a]anthracene‚ Fluoranthene‚ Fluorene‚ Indeno[1‚2‚3-cd]pyrene‚ 3-Methylcholanthrene‚ 2-Methylnaphthalene‚ Naphthalene‚ Perylene‚ Phenanthrene‚ Pyrene)‚ 11 benzene derivatives (Acetophenone‚ Aniline‚ Benzoic acid‚ Benzyl alcohol‚ 2‚4-Dimethylphenol‚ Hydroquinone‚ 2-Methylphenol‚ 3-Methylphenol
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BIOREMEDIATION Bioremediation is such type of technology in which microorganism‚ fungi‚ bacteria‚ plant and there is use to convert polluted condition in to original condition. Through bioremediation process microorganism act on pollutant or on chemicals due to which pollution occur and help that thing to come back in its original condition. Bioremediation is an option to offers the possibility to destroy or renders various harmful thing through natural biological activity.
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