November 5‚ 2014 Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA
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Extraction of Caffeine from Tea leaves Casinabe‚ Antonio Karlo‚*Catahay‚ Jesus Alfonso‚ Cerdena‚ O’neil‚ Cordova‚ Robelle Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España Street‚ Manila 108 Group 4 2MB Date Submitted: December 13‚ 2012 ------------------------------------------------- ABSTRACT: The purpose of this experiment is to extract the amount of caffeine present in tea bags. In this experiment‚ isolation‚ characterization and purification
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Discussion: Extraction and crystallization- The purpose of this lab was to extract and crystallize compounds in a mixture using acids and bases. 1 gram of the unknown was dissolved into 30 mL of ethyl acetate. The mixture was then transferred to a separatory funnel. Using aqueous solutions of different pH we were able to separate organic acids‚ bases and neutral compounds from each other. Using three 10 mL portions of HCl‚ we reacted the HCl with the base in solution. This made it possible to
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DNA EXTRACTION In extracting chromatin from the cells of wheat germ there are seven steps to follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes have nucleus membrane-bound organelles‚ while prokaryotic does not. The polyploidal eukaryotic cell has DNA that is held in the nucleus while the prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is more complex and extensive than the other. Prokaryote is a bacterial cell that
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Objective: To extract caffeine from tea powder using polar - nonpolar solvent extraction technique. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. The solution of these dissolved compounds is referred to as the Extract. Here the organic solvent dichloromethane is used to extract caffeine from an aqueous extract of tea leaves
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Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh‚ Soft Fruit (e.g.‚ Grapes‚ Strawberries‚ Banana‚ etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride‚ detergent and waterFor ice cold ethanol‚ store in the freezer 60 minutes before use. Procedure If you have not done so‚ prepare the ethanol by placing it in a freezer for approximately
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Extraction of Eugenol from Cloves 02/01/2014 CHM2210L University of North Florida Abstract Eugenol is found in the essential oil of cloves and has distinct properties that make it an important product to both food and drug industries. (Bhimrao‚ et. al‚ 2004). To utilize many of eugenol’s characteristic properties‚ it is necessary to isolate this organic chemical from the other components of cloves. In this experiment‚ eugenol was isolated from a sample of cloves using a series of techniques
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EXTRACTION OF METALS SUMMARY Most metals exist in compounds in rocks in the Earth’s crust. Many of these ores contain oxides or sulphides of the metals. The compounds need to be reduced to produce the metals as elements. Metals are very useful to us because of their special properties. Reduction means removal of oxygen or gain of electrons. Carbon is a good chemical reducing agent because it readily combines with oxygen‚ but it is only useful for the metals below it in the Reactivity
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around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides are composed of 5 carbon sugar attached to the phosphate group and a nitrogen containing base. The base might be Adenine‚Cytosine‚Guanine‚or Thymine. Nucleotides are linked together by covalent bond through the sugar and phosphate. The
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Experiment 2: Extraction – Evaluation Summary Labs reports must be typed and chemical structures must be drawn with ChemDraw. Report must not exceed three pages (including this page). Page limit does not include any attached spectra or references. ** Deductions for hand written report/structures and exceeding page limit ** Report Breakdown Data/Results: _________________ /10 Discussion: _________________ /10 Report Total: _________________ /20 Other Lab Marks
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