3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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with Particular Emphasis on Political Participation and Leadership Report of the Expert Group Meeting Addis-Ababa‚ Ethiopia 24 – 27 October 2005 Division for the Advancement of Women Department of Economic and Social Affairs 2 UN Plaza‚ 12th Floor New York‚ NY 10017 Fax: (212) 963-3463 Email: daw@un.org Website: http://www.un.org/womenwatch/daw TABLE OF CONTENTS Pages I. II. Introduction……………………………………………………………..………………... Organization of work…………………………………………………..………………… A. B. C. D. E. F. III. Participation…………………………………………………
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Reconstruction did not successfully solve the problems caused by slavery and the Civil War because the slaves were still being tricked and the white people were discriminating against the African Americans. Slavery was a big issue in the United States. They took advantage of the African Americans during the Civil War. Lincoln believed that slavery was wrong and if he set the slaves free during the Civil War‚ then they would get a huge advantage and reach the end of the war with the Union’s victory
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possibility of migration‚ as many people come and go‚ the group may have finally dwindled down to nothing. They could’ve starved to death or just died from disease or infection as the immunizations were not common back in these days. References: AIU-Online.com‚ (2010). Indigenous People and the European Explorers in South America‚ From M.U.S.E. Retrieved November 25‚ 2010
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The Reconstruction Act of March‚ 1867 reorganized the South into five military districts‚ each under control of a Union general. The Act required universal male suffrage to elect delegates to conventions‚ required granting the vote to freed men‚ and the ratification of the 14th Amendment. The term “Negro Rule” came from the enfranchisement of freedmen and their participation in state and local government.16 Enfranchisement and office holding allowed another change in Southern politics that white
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"The Leader of the People" In John Steinbeck’s "The Leader of the People‚" a theme that is portrayed throughout the story is that of the contrast between dream and reality. In the story‚ Grandfather cannot come to grasp that his dreams of the West and moving across the plains are over and that reality has set in. Another story that contrasts dream and reality is Ambrose Pierce’s "An Occurrence at Owl Creek Bridge." In this story‚ Farquhar cannot tell the difference between dream and reality until
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China Southern Airlines was founded in 1995 and is based in Guangzhou. It is China’s largest airline in terms of fleet size‚ with 309 aircraft. The airline employees over 45‚500 full time employees. Its main hubs include Guangzhou and Beijing‚ but it operates in over 150 cities throughout China. Furthermore‚ China Southern operates 616 domestic 82 routes‚ including 90 international routes and 25 routes in Hong Kong and Macau. As China’s only company that is in world top ten passenger airlines
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Transactional Leader as the word implies business or exchange. Burns‚ 1978‚ opined that a transactional leader is someone who leads though social exchange. It is a process that I simply call trade by batter‚ you do this for me‚ and you get that in return. A politician can be a transactional leader when he seeks to exchange one thing for another i.e. making promises and pulling through with the promises after an election. Also most business leaders are seen as transformational when they give out incentives
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• Beringia- a subcontinent bridging Asia and North America‚ named after the Bering Straits. • Athapascan- A people that began to settle the forests in the northwestern area of North America around 5000 B.C.E. • Clovis tradition-A powerful new and sophisticated style of tool making‚ unlike anything found in the old world • Pleistocene Overkill- Intensified hunting efforts brought on in response to lowered reproduction and survival rates of large animals • Archaic period- the period
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Reconstruction The reconstruction was followed by the civil war and took place in the southern states. The reconstruction was an attempt of bringing in confederate states back into the union. Amendments were added to the constitution to protect black people in the south. This was important because they were trying to make the south safe for the blacks. Industrial Age The industrial age took place in the 18th and 19th centuries. The industrial age began in Britain and took a widespread
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