The purpose of the experiment was to find out how temperature affects the enzymes activity. For example‚ in Humans if the temperature is too high‚ the individual’s brain enzymes can denature and cause life threatening problems. The opposite can occur as well‚ if the temperature is too low‚ hypothermia can occur and it can be dangerous (Wilson‚ 1996). In the experiment optimal conditions for fungal and bacterial amylase was measured as well. Discovering information‚ such as the optimal temperature
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Although the reproduction of harmful bacteria may be hazardous‚ it is necessary for microbiologists to grow high populations of bacteria in the attempt to study the causes and provide prevention methods for spreading harmful bacterium. The purpose of this lab was to collect and observe microorganisms from the environment and the human body and placing into the appropriate media using aseptic transfer techniques. The necessity and value of collecting‚ identifying and analyzing is critical for learning how
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Purpose: The purpose of this lab was to see how the changes in exercise intensity affect the rate of metabolism within the body. Methods: In this lab‚ indirect calorimetry was used to measure metabolic rate by calculating caloric expenditure by the measurements of oxygen consumption. The variables measured were the fraction of oxygen expired‚ the fraction of carbon dioxide expired‚ and the total volume of air inspired. This experiment required the participation of two volunteers. First‚ the variables
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Botany Lab ------------------------------------------------- Introduction This study observed the effects of different body fluids and solutions relative to breaking down bacteria‚ specifically in the human body. The enzymes we studied‚ lysozomes‚ help the body lyse‚ or break down bacteria by targeting peptidoglycan in bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Title: Effects of Light on Photosynthesis Introduction: Photosynthesis is the complex process by which carbon dioxide and water are used to make carbohydrates by using light energy in green plants. The objective of this experiment is to measure the rate of photosynthesis Hypothesis: The petri dish that is exposed to the most light and with the NaHCO3 solution will have the best results and the petri dish that is kept in the dark will have the poorest results. Material and Methods: 1. Get 4
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Arielle Fisher GFP Lab November 16‚ 2011 Figure 1 A. B. C. D. Figure 1. Confocal images at 400x magnification of HeLa cells to locate GFP activity. HeLa cells were (A) tagged with Green Fluorescent Protein (GFP) (B) labeled with GFP and 14.13 µl Dexamethasone (C) tagged with GFP fused to the glucocorticoid binding
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EXPERIMENT Materials 200 Toothpicks Timer Tape Controls 50 toothpicks per trial. 120 seconds per trial. The same brand of toothpicks. One toothpick broken at a time (except for Mutation Trial 2). One toothpick broken into two pieces equals one reaction. Broken toothpicks cannot react again. (Toothpicks can only be broken once) The toothpicks are broken between the thumb‚ index‚ and middle finger (toothpickase). Break two toothpicks at a time (Trail 3). Tape the index finger and thumb. (Trail
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obtained • Temperature of the surrounding How the variables can be controlled: • Equally sized daphnia should be used in the experiment • All daphnia should be collected at a same place‚ in uncontaminated waters • Experiment should be carried out in a lab at room temperature Apparatus/materials: • Pipette • Petri dish • Filter paper • Silicon grease • Needle • 0.1%‚ 0.2%‚0.3%‚0.4%‚0.5% of caffeine solutions • Daphnia culture Methodology: • Select a large specimen and‚ with a pipette‚ transfer
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Enzymes are catalysts that are used to speed up chemical reactions. Adding inorganic catalysts to reactants increase the rate of most chemical reactions. However‚ all enzymes are different and there are numerous amounts. Many enzymes are essential for life and reactions would not happen rapidly to maintain life with the help of enzymes. Specific enzymes lower the activation energy for specific reactions and shapes. Activation energy is required to start a chemical reaction. This occurs when energy
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