stranded with base single stranded pairing Helix Yes No Similarities: Nucleotides are basic structural unit Complementary base pairing by hydrogen bonds Both have a pentose sugar and phosphoric acid E. Protein Synthesis Demonstrate a knowledge of the basic steps of protein synthesis‚ identifying the roles of DNA‚ mRNA‚ and ribosomes in the process of transcription and translation Transcription RNA molecule makes complementary copy of DNA RNA goes into cytoplasm Translation Initiation:
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EXTRACTION AND ISOLATION OF PROTEINS Background of the experiment Pure proteins are not readily available for study or use. There are certain methods that should be done in order to obtain proteins from its sources. The objective of this experiment is to isolate casein from milk and albumin from egg and to identify and explain the principles behind the methods employed for protein extraction. Results and Discussion There are many possible sources where we could get proteins. It can be from plants
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white. It is because the protein in egg white undergoes denaturation‚ the cross linkage(the hydrogen bonds‚ ionic bonds and disulphride bonds) which maintain the protein shape destructed‚ so protein lose its tertiary conformation. This denaturing process is very important‚ because before protein can be used in digestion they must be unfolded. Part A: denaturation of egg white Aim: To examine the factors on the effect of denaturation of egg white. Principle: As protein denaturation can be cause
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Abstract The activity of invertase and the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved
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* Training the class on how to make a tasty protein shake * Main interest is working out and staying active * Before I get any further I want to list the training objectives and they are the process and ingredients used to make a shake‚ the benefits and common mistakes. * Just get a general view of the audience‚ HOW MANY HAVE TRIED WHEY OR SOY BASED PROTEIN BEFORE? AND YOUR EXPECTATIONS ON THE TASTE? * What you need to make a protein shake? Why Banana (sweet when ripe and added potassium
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During protein digestion‚ a water molecule is added which breaks down the carbonyl-carbon-nitrogen sin the peptide bonds of proteins causing the liberation of amino acids. This process is referred to as proteolysis. Water molecules break down the carbonyl-carbon-nitrogen single bond (peptide bond) that holds single amino acid molecules together (Caroline Ritchie‚ 2013. This process is called hydrolysis and is catalysed by protease. The three main protease enzymes produced during digestion are pepsin
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Protein Synthesis Notes If DNA’s shape is altered it can’t function properly. RNA is more flexible so changing the shape changes the function‚ which is wanted. Any shape of RNA can become another. Three shapes/functions of RNA: -mRNA: messenger (linear_____) -rRNA: ribosomal (large and like a globe) More stable than mRNA. -tRNA: transfer (shaped like t) If you flatten this it becomes mRNA. Hydrogen Bonds ………………………………. Protein Synthesis Overview- Two Processes: Transcription- synthesis of
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The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff methods
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The Tensile Behaviour of Common Engineering Polymers Abstract The mechanical properties of polymers vary significantly from polymer to polymer as a result of atomic structures and bond strength. The tensile properties of representative samples from the common polymer were tested and evaluated in this experiment. This laboratory experiment is also designed to demonstrate the procedure used for obtaining mechanical properties as modulus of elasticity‚ yield strength‚ ultimate tensile strength
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of Muscle Proteins to Infer Evolutionary Relationships of Mammals‚ Fish and Birds Abstract This experiment used electrophoresis to examine the makeup of muscle proteins from two mammals (cow‚ Bos taurus; bear‚ Ursus americanus)‚ two aves (chicken‚ Gallus gallus; turkey‚ Meleagris gallopavo)‚ and two fish (King Salmon‚ Oncorhynchus tshawytscha; Albacore Tuna‚ Scombridae unclassified). The hypothesis was that as species have diverged evolutionarily‚ the makeup of their muscle proteins has also
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