Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A
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temperature of different polymers 8.1 Compare and comment on the results obtained. LDPE has the lowest average melting temperature (103.6°C) out of the 4 polymers. It is because LDPE is a linear polymer that has a branched structure which is able to expel the applied heat faster through the “branches” and melt faster. HPDE has the second lowest average melting temperature (139.5°C) out of the 4 polymers. Unlike LDPE‚ HDPE does not have a branched structure and the HPDE polymer chains are able to pack
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(ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of charged particles such as proteins when placed
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Carbohydrates‚ Proteins‚ lipids‚ and nucleic Acid Lab Exercise 6 Date: 9/17/12 Bio 102-11 Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound. Background Most organic compounds in living organisms are carbohydrates‚ proteins‚ lipids‚ and nucleic acids they are called macromolecules
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Project 1: Polymer Ball Vivian Kim General Chemistry 2045L Introduction Our assignment in this project was to create a bouncy ball for a company using polyvinyl alcohol (PVA)‚ polyvinyl acetate solid and sodium borate‚ by varying the ration of ingredients and to determine the best recipe for our bouncy ball. The company chose the materials because of their odorless and nontoxic properties. PVA is a water-soluble synthetic polymer and has adhesive properties
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The Some of the roles proteins play in the human body like Proteins serve as enzymes‚ help maintain the body’s fluid balance by attracting water‚ help maintain acid-base balance by acting as buffers‚ act against disease agents as antibodies‚ regulate body processes as hormones‚ transport nutrients and other molecules into and out of cells‚ help clot blood‚ help make scar tissue and bones‚ and serve as light-sensitive visual pigments. Also‚ Growth and repair this the most important and Individual
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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In the past protein powder has been the main product of choice of bodybuilders and other weightlifters who spend all of their time in the gym with the goal of becoming as strong and muscular as they can. But‚ in the past years‚ many other athletes and people have discovered the many benefits that protein powders have‚ causing the popularity of this product to increase rapidly. Many studies have proven that using protein powder while performing a resistance exercise plan will increase overall muscular
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Background: Pepsinogen is an inactive protein that is the mucus lining of the stomach. When converted to pepsin‚ the enzyme is used to break down large or undigested protein they has been absorbed by the small intestine. The digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web
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