________________________________________________________________________________ ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of total nitrogen in food and crude protein calculation (Kjeldahl method) ___________________________________________________________ Responsible person: Assoc.Prof. Ing.Kateřina Riddellová‚ Ph.D. CONTENT ______________________________________________________________________________________________________________________ Required
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assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds of plastic (polystyrene or polyvinyl chloride)‚ usually by hydrophobic interaction. The steps of the ELISA procedure are simple to perform. There are mainly FOUR phases in ELISA: 1. Coating
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Food1120 Lecture: Foods in the News Patrick Spicer p.spicer@unsw.edu.au APril 16‚ 2014 Topic: contemporary coverage of food topics • Your area of study is central to people’s lives and will thus be a regular topic of conversation and news. • As a trained scientist‚ you will have knowledge and experience these people don’t. You must use it well for your own benefit and others’. • We will talk today about how to read and assess news coverage of food and other scientific
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energy levels‚ positive inspiration to others and the potential for a heightened quality of life. Weight Maintenance Following a healthy eating plan -- one which emphasizes many fruits and vegetables‚ whole grains‚ low-fat dairy products and lean proteins‚ as well as low levels of saturated and trans fats and low cholesterol‚ sodium and sugar -- contributes to weight maintenance. Staying within your recommended daily calorie intake and eating moderately sized meals also helps you maintain a healthy
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What are major characteristics of traditional Japanese food‚ and how has it been influenced by geography and religion? Japanese cuisine has developed over the centuries as a result of many political and social changes throughout Japan. Japanese cuisine is known for its emphasis on seasonality of food‚ quality of ingredients and presentation. Japanese food is well known all over the world for its low fat and nutritional values. The Japanese food might appear bland and less spicy because they don’t
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IS FAST FOOD IS GOOD FOR OUR HEALTH OR NOT In the busy and hectic schedule of modern times‚ people have no time to prepare nutritious food during the day. They have to walk out early in the morning and spend most of the time at workplaces. Thus‚ they have to depend on the instant foods that are available in restaurants and hotels. This is the prime choice of youngsters for the delicious and tempting taste of junk food. In spite of dangers of fast food‚ they appeal to everyone’s appetite irrespective
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traditions of cooking. They take pride in their food and live by the credo that food is only used if it is fresh‚ local and in season. There has been much debate on what defines Italian food and through research one can see that Italian food is not typical of a specific dish‚ rather that each region offers a new variance of depth and originality that is not common to the Americanized beliefs of solely pasta and pizza. Yes one could lump Italian food into categories such as breads‚ pasta‚ olive oil
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Food Shortage. Lester Brown addresses this issue on chapter nine of his book Plan B. He tries to explain what can be done to sufficiently feed a world with a population of 8 billion persons. He gives an example of China’s way of increasing its food production and how in just about a decade‚ china was able to have enough food to feed hundreds of millions of people who were starving. China became self-sufficient in food production and in 2005 was able to manage its hungry population and she not
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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