3.7.2.1.5 Urea test PRINCIPLE Urea is hydrolyzed in the presence of urease to produce ammonia and CO2. The ammonia produced combines with 2 – oxoglutarate and NADH in the presence of GLDH to yield glutamate and NAD. Urea + H2O + 2H+ 2NH4+ + CO2 NH4+ + 2-Oxoglutarate +NADH H2O +NAD+ + Glutamate The decrease in absorbance due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia
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Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
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Description for Using Malaria Rapid Diagnostic Test (mRDTs) The procedure of the test as contained in the manufacturer’s instructions as approved by the National guidelines for diagnosis and treatment of malaria‚ (FMoH‚ 2011) will be followed. its reliability was futher proven by Olaniyan‚ et al.‚ (2013). The RDT provided for the study has LOT number and expiry date which must be recorded. Preferably RDT of known quality assurance will be used. The device usually contains a cassette‚ an applicator
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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Cold pressor test is widely used as test for auto- nomic functions. It has been used as diagnostic test for diseases like hypertension and other cardiac auto- nomic disorders. During cold pressor test ‚ the hand or foot is immersed in cold water bath held at a con- stant temperature at 80 C. Cold water immersion activates afferent pain and temperature neurons‚ re- sulting in a centrally mediated stimulation of sympa- thetic efferent neurons. In healthy subjects ‚ the CPT causes increase
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the central analgesic activity of AVS022 and its components on hot-plate test Initial studies utilizing the hot-plate test
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1. Both of the subjects in this test preformed excellent compared to the rankings provided. Victors Mean Power put him in between the 85 to 90 percentile with his score of 639.83 and his Anaerobic Capacity put him in between the 60 to 65 percentile. These scores indicate that Victor trains more with resistance than aerobic type exercises because of the high score of his Mean Power. These scores also correlate with his VO2 Max test we preformed earlier. His VO2 Max of 3.4 is low compared to the high
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4 gram stains‚ the slide was blot dried and observed under microscope at 100x with oil immersion. The slide either appear in pink (gram-negative) or purple (gram-positive). Catalase Test: Catalase test enzyme detection of microorganisms‚ catalase hydrolysis hydrogen peroxide (H2O2) into H2O and O2. The catalase test was performed by used a sterile transfer loop been flamed to remove small amount of bacteria from slant agar and placed on the slide. Then
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Abstract: The purpose of this lab experiment was to discover genetically modified organisms. We analyzed our results and found that our sample was GMO positive. These results were attained through polymerase chain reaction and agarose gel electrophoresis. We came to the conclusion that the presence of a band in lane 4 confirms that there are indeed genetically modified organisms. Introduction: Overall‚ the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in
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load test Aim: To determine the strength characteristics of a rock using the point load test and visual judgement. Materials Loading device Loading frame Pump Ram Platens Rock Caliper Method (Standard‚ 2007) 1. Measure the dimensions of the rock which includes the diameter and length 2. Find the length to diameter ratio to determine which test will be used. If the ratio is greater than 1 use diametrical test. But if the ratio is between 0.6 and 1 use axial test 3. The axial test was
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