(NH2CH2CH2NH2) that formed [Co(en)2(H2O)2]Cl. Then the [Co(en)2(H2O)2]Cl was reacted with H2O2 to change the cobalt’s charge to cobalt(III) from cobalt(II). Finally HCl was added into the solution to form [Co(en)2Cl2]Cl. The percent yield was 45.51%.To synthesis trans isomers of [Co(en)2Cl2]Cl‚ distilled water was first mixed with CoCl2▪6H2O‚ the solution color was changed to wine color. After ethlenediamine (NH2CH2CH2NH2) was added‚ the color of the solution changed to blood color‚ and kept its color until
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of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis. The process of the PCR is complex and contains different components and reactions. The first main step of PCR is denaturing. The temperature at which
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Unit 4 Assessment 2-Benzoic Acid Synthesis Synthesis and Investigation of Benzoic Acid Our aims: Create benzoic acid using benzaldehyde and hydrogen peroxide. Then remove some impurities from the benzoic acid crystals. Apparatus: Titration Pipette (25 cm3) Burette (50 cm3) Retort stand Clamp Conical flask (250 cm3) Volumetric flask (250 cm3) and stopper White tile Beakers (250 cm3) Dropping pipette Filter funnel Deionised water Phenolphthalein indicator Volumetric flask
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Objective: The objective of this lab is to determine and analyze the many chemical qualities of copper ‚ by using many solutions that enable copper to undergo chemical changes that will take copper from a metal back to its original state. This is done using cooper‚ Concentrated HNO3‚ 6 M NaOH ‚ Bunsen burner‚ 6 M H2SO4‚ Mg ribbon‚ and two test tubes. The techniques used in this lab which is cleaning glassware‚ disposing of chemicals ‚ measuring mass ‚ centrifugation ‚ venting gases‚ and test tube
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References 1.) Lehman‚ John. Operational Organic Chemistry‚ 3rd Edition. Experiment 4 (pp. 38-45; 680-687; 644-650)‚ Prentice-Hall‚ 1999. 2.) Laboratory Reference Manual: Experiment 3. Retrieved from: http://academic.reed.edu/chemistry/alan/201_202/lab_manual/expt_salicylic_acid/background.html 3.) Lab 5: Synthesis of Salicylic Acid. Retrieved from: http://academic.evergreen.edu/curricular/whatscookin/Lab%20five%20%20methyl%20saliclate.htm 4.) Handout: Synthesis of Salicylic Acid. Retrieved from:
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INTRODUCTION: Penicillin is the basis for most of the antibiotics in the world just as aspirin is the basis for many drugs. Therefore‚ if there had been only two drugs to invent‚ it would be down to penicillin and aspirin. Hippocrates‚ the father of modern medicine lived sometime between 460 B.C and 377 B.C. Hippocrates has left historical records of pain relief treatments‚ including the use of a powder made from the bark and leaves of the willow tree to help heal headaches‚ pains and fevers.
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Pat 1 Synthesis of 4-aryl-6-indolylpyridine-2-carbonitriles and evaluation of their biological activity Synthesis of 6-indolyl-4-aryl-3-cyanopyridine-2-one (1a-e) Employing the MCRs approach and microwave irradiation‚ an equimolar amounts of aromatic aldehyde‚ 3-acetylindole‚ ethyl cyanoacetate were reacted with excess of ammonium acetate in one-pot‚ reaction‚ under the microwave irradiation‚ afforded a novel series of 4-aryl-6-indolyl-nicotinonitrile-2-one derivatives 1a-e. Ethylene glycol was
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solution with a known concentration of 4.047x10-3 M was obtained. A 250-mL sample of a 10-fold dilution of this ionic iron solution and 5 drops of concentrated HNO3 was made. This solution served as the source of ionic Iron for the remainder of the lab and was labeled “stock ionic Iron solution.” Next‚ a 50-mL aqueous ionic Iron and FerroZine® complex solution was prepared by adding 5.00mL stock ionic Iron‚ 3-mL of acetic acid buffer‚ 2-mL of 5% hydroxylamine hydrochloride‚ allowing five minutes for
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Tarnas 1 Kulananalu Tarnas Professor Kirk Organic Chemistry Lab 8 March 2014 Project 4: Interconversion of 4-tert-butylcyclohexanol and 4-tert-butylcyclohexanone Intro: It is truly incredible how the smallest differences between molecules can lead them to react and behave entirely differently. These differences are perhaps most highlighted in the medical field where stereochemistry of a molecule can be the difference between having a medically active‚ effective drug and a toxic‚ potentially
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