University of Santo Tomas Faculty of Pharmacy EFFECT OF pH ON ENZYMATIC ACTIVITY Abogado‚ Paula Isabel‚ Agustin‚ Nikka Rebecca‚ Andres‚ Mary Anne B.‚ *Austria‚ Beatrice April‚ Baybay‚ Jan Miko Aaron 2BMT I. ABSTRACT pH greatly affects the activity of enzymes. There is a point or pH level where maximum activity of enzyme can be achieved‚ this is called optimum pH. Invertase was extracted from yeast and used as the detanured enzymes. Two sets of six test tubes were each added with different
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The experiment consists of 5 steps—Inoculation‚ fermentation‚ cell lysis‚ inclusion body solubilization‚ and peptide purification. In the first two steps‚ E coli with Snake 6 peptides is cultivated. Ultraviolet–visible spectroscopy (UV-vis) is used to quantify bacteria through detection of their optical density (O.D.). After reaching a certain concentration in the fermentation batch‚ inclusion bodies (IBs) are extracted from these E coli by adding Bugbuster lysis solution and lysozyme. Polyacrylamide
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Synopsis The purpose of this experiment was for students to prepare some simple organic compound and to purify the compound by recrystallisation. This experiment allowed the students to conduct the synthesis of aspirin‚ acquire the skills of recrystallisation using normal filtration and reinforce the technique of the melting point determination. The method used to synthesize aspirin was esterification by reacting salicylic acid with acetic anhydride with the presence of dehydrating agent‚ conc
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Ellen Grant Honors Bio I Organic Compounds Problems and Objectives: The purpose of this lab was to select the suitable test for an organic molecule and define the safe process for that test. An organic molecule is normally found in or produced by living systems. (Unknown Author‚ 2014‚ www.biology-online.org/organic_molecule)In this lab one was supposed to infer the results of the test and be able to identify if major biomolecules are present. You were to analyze a variety of tests including
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Synthesis of Isopentyl Acetate (Banana Oil) Using A Fischer Esterification Reaction Purpose: The purpose of this experiment is to synthesize Isopentyl Acetate using a Fischer esterification reaction. Fischer esterification is an acid-catalyzed condensation of an alcohol and a carboxylic acid‚ yielding an ester and water. Isopentyl Acetate has the scent of banana oil‚ once synthesized it will be purified by distillation. Then the analysis of the sample using H NMR and IR will determine purity
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b. Characterization of Aspirin B. Set – up Fig. 11.1. Suction Filtration Set – up C. Table 11.1 List of Necessary Chemicals Sample Structure Purpose Physical Properties Hazards Salicylic Acid Starting Material Appearance: white powder Molar Mass: 138.12 g/mol Melting Point: 1590C Boiling Point: 2110C Density: 1.44 g/cm3 Mildly irritating (pulmonary irritants). Harmful by inhalation‚ ingestion and through skin absorption. Acetic Anhydride
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Organic Chemistry Laboratory Report. Experiment 1: Crystallization. Objectives: 1. To study the crystallization process. 2. To identify the best suitable solvent to use for the crystallization process. 3. Gain an experience in purifying an organic compound by the techniques of the crystallization. Introduction. Crystallization is a technique which chemists use to purify solid compounds. It is one of the fundamental procedures each chemist
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buffer at pH 7.0‚ the CA-2Pb2+ formation resulted in a distinct color change from red to blue‚ accompanied by a remarkable bathochromic shift from 527 nm with a shoulder at 558 nm to 605 nm in the absorption spectrum [28]. Since there have been some reports on the complex formation between Cys and Pb2+ in an aqueous solution [30‚ 31]‚ the sensing selectivity of CA-2Pb2+ toward Cys was investigated by absorption spectroscopy. The color changes and absorption spectral study of CA-2Pb2+ (50 μM) were performed
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14 may be a possible EEC. Moreover‚ the calculated compensation temperature by inverting the straight line slope‚ T comp = 57.9 ºC‚ is higher than the average experimental value‚ T exp = 32.5 ºC. This great difference between these two values of temperatures might reflect the existence of real EEC rather than experimental error [58]. Another evidence of EEC is the approximate constant ∆G # with a significant change in both ∆H # and ∆S # . Finally‚ EEC was also investigated by using
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Abstract: This experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”. The first part of experiment was the synthesis of L-phenylalanine methyl ester hydrochloride. The methyl ester can be synthesised by reaction of thionyl chloride‚ SOCl2 and dry methanol with L-phenylalanine under reflux condition. The peptide bond was formed later in the experiment‚ where HBTU‚ DiPEA and a solution of Fmoc-Gly-OH in DMF were added to a solution of L-phenylalanine methyl ester hydrochloride in DMF
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