University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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most pennies if we were to tape them to two stools 16 cm apart‚ dropping pennies into a cup tied around the noodle? Expiremental Variables - We will be able to identify the mass of a penny and how it can test the strength of a pasta noodle as a result of the downward pull of gravity on the penny. Test Variable: The type of noodle is what I’d be changing throughout the process Outcome Variable: I would be observing how many pennies each noodle could hold counting every single one that drops into the
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Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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In fermentation the pyruvate is converted into ethyl alcohol‚ through the oxidation of the 2 NADH molecules‚ which returns them to two NAD+’s (Freeman‚ 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested
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The normal breathing test tube took the longest then after hyperventilation‚ and then after exercise had the biggest CO2 concentration because of how quickly it was detected. See Table 1. In the second experiment‚ the original pH of the alkaline phenol red was 4.0. After
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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Potato Osmosis and Diffusion Lab Report Emily Cocq Research Question: Will there be a difference in the experimented potato cores after being subjected to high concentrations of sugar? Introduction: Water can move through protein channels in cell membrane/cell walls. The water will move due to the water potential of the cells. If there is a greater concentration of solutes (chemicals) inside the cell than outside the cell and the chemicals can not move‚ then water will respond by moving
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Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of
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