Abstract: The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically‚ a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin‚ an antibiotic. Essentially‚ we wanted to determine the conditions of the bacteria that would glow.
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2. FORMAL LETTER-COMPLAINT Rajvind Gill‚ 10‚ Jalan Jujur‚ Taman Jayadiri‚ 68000 Ampang‚ Selangor The Director‚ Ampang Jaya Municipal Council‚ Bangunan Mentari‚ Ampang Jaya‚ 68000 SELANGOR. 15 SEPTEMBER 2009 Dear Sir‚ Poor Maintenance of Taman Jayadiri I am writing this letter on behalf of the residents of Taman Jayadiri to complain about the dismal conditions we have been putting up with for the past three months. 2. One of the main grouses of the residents concerns uncollected rubbish
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Short version Alice part Adaptivity of architecture T: City is something that changes and grows. Cities become inevitably dysfunctional and need maintenance‚ otherwise they will decade or die off. We assume that cities are only about thriving and growth‚ but actually it is more about decade‚ declining and abandon of the cities and the history and memories of them. We like to believe that buildings are permanent‚ totally functional and save‚ however this might be one of the biggest lies that have been told in architecture schools
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opportunities in branching out and looking for a career path. In general I would say that it takes a great deal of self-motivation in choosing a lifestyle for ourselves with such little “big-world” experience‚ however‚ for those of us who chose to ask for assistance from our teachers and mentors‚ the task proved to be relatively easy. I had no clue which path I wanted to follow or where it would take me although I had toyed with several career choices ranging from a graphic designer to a lawyer. It
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self-educate one’s self‚ and many people loved to use letters for self-expressing‚ critical reading and for exchanging ideas with like-minded people. Letters were also used as a form of communication between two people and so there exists two types of such letters‚ and they are: 1. Formal Letter: Formal letters are letters‚ written to a more authoritative person like‚ from a student to a teacher‚ from a normal citizen to the newspaper editor‚ from a fan to his
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the revenue generation * Inadequate system to monitor the expenses * All the expenses should go through an approval process which requires all the expenses should be properly documented and the need explained. This is not in place in Choice House 2. Identify ethical issues that Ashley should discuss with the Finance Committee? Which IMA Ethical Standards would apply to Ashley? Discuss. * Credibility: The ED should record all expenses and provide all information when I asked
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4100 CONTRACTUAL CHOICE Scott E. Masten Louis and Myrtle Research Professor of Business and Law‚ University of Michigan Business School © Copyright 1999 Scott E. Masten Abstract This chapter discusses alternative theories of contract choice and design with special emphasis on (i) the interaction between contract design and contract enforcement and (ii) the explanatory power of alternative theories. After discussing the primary functions of contract‚ the entry reviews the assumptions
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Assume the CAVA confirms the existence of an unavoidable conflict. The congruent coloring requires the fixed color be chosen‚ and consequently the two top rims be congruent conflict free. The focus is on these two issues. Congruence of Two Vertices Within three colors‚ two vertices (p‚ q)V(G) can be in one of the congruence relation defined as: congruent c(p) ≅ c(q)‚ the resulting color c(q) always equals the selected color for c(p). anti-congruent c(p) c(q)‚ <p‚ q>E‚ the resulting color c(q)
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’TWO HANDS’ Released in mid 1999‚ ’Two Hands’ was a box office success in Australia. Financed by the Australian Film Finance Corporation with assistance from Film Victoria and the New South Wales Film and Television Office. The film was made on a total budget of $4.4 million. ’Two Hands’ went on to make $5‚478‚485 at the box office‚ it was the first Australian film to open in top position at the Australian box office within the last six years prior to its release. With a total budget of
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has had a remarkable impact on post-modern society. It is the first of its kind‚ in that a cartoon is portraying a realistic nuclear American family. The show was first created as a series of short skits by Matt Groening for The Tracy Ullman Show. Two years later in 1989‚ The Simpsons became a primetime hit for the FOX Broadcasting Network. Fast forward to 2012 and the show has become a billion
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