BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
Premium Reaction rate Chemical kinetics Rate equation
Many Americans have raised interest about gluten as the label “gluten free” has been seen more frequently. In fact‚ about 30% of Americans have recently made the switch over to a gluten-free diet. Gluten free diets have mainly been designed to treat celiac disease. About 1% of the population has been diagnosed with celiac disease‚ meaning they have trouble digesting gluten‚ a protein in grains such as wheat‚ rye‚ and barley. These components are often found in beer‚ pasta‚ and bread. If only 1% of
Premium Nutrition Food Obesity
Enzyme Catalysis Abstract: Catalysis is an enzyme that decomposes hydrogen peroxide into oxygen and water. In this lab we will conduct a series of experiments to determine the affects of; pH‚ temperature‚ and concentration change on such catalysis. After completing the experiments we determined that catalase works most efficiently when in a 27°c‚ 50% (5 ml of catalase – 5ml of water)‚ and a normal body pH of 7°. By: Patrick Jawien Course Code: SBI 4U Performance Date: 25 September 2012
Premium Catalase Enzyme
Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
Free Catalysis Chemical reaction Chemical kinetics
introduction to enzymes Enzymes are the foundation of energy and the life force in all living things. They are responsible for building‚ detoxifying‚ and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes also regulate tens of thousands of other biochemical functions that take place in the body every day. Without enzymes‚ seeds would not sprout‚ fruit would not ripen‚ leaves would not change color‚ and life would not exist. Therefore‚ the study of enzymes has
Premium Chemical reaction Chemistry Metabolism
The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
Premium Enzyme
Enzymes control the rate of metabolic reactions‚ they act as biological catalysts‚ which means they are used but not used up and they also control the speed of the reaction. Enzymes are proteins which means that anything that disrupts this structure such as high temperature or change in pH will affect the enzyme activity. There are many factors affecting enzyme action for example temperature effects them‚ if the Increasing the heat gives molecules more kinetic energy so they vibrate this can then
Premium Enzyme Enzyme inhibitor Metabolism
Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case‚ H2O2 will produce more O2‚ in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from
Premium PH Enzyme
The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
Free Enzyme Starch
Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in the cells of the body
Premium Oxygen Catalase Enzyme