Ahmad Shrab . Introduction This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition‚ learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab ‚ I worked two experiments ‚ the first one is cultivation bacteria "colony "‚ and When microorganisms are cultivated
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Egg Lab Report Introduction: An egg is a model of a human because the egg has a cell membrane like humans do inside and outside of the body that let things pass through like water. We can use eggs to study the effect of changes in the external environment on the internal environment by having harsh environments like putting the egg in only alcohol and see what happens to the inside of the egg. Diffusion is the movement of a substance down its concentration gradient from a more to a less concentrated
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one outlier that was strange was the one paper towels. Only 14 of the 15 seeds germinated in that group. The difference that we could observation was the healthiness of the plants. IV. Conclusion Paragraph 1 Recap (Evidence): The purpose of our lab was to see if the amount paper towels would affect the germination of the seeds. My hypothesis was‚ if I increase the amount of paper towels‚ then the germination rate would increase too. We observed the petri dishes in class and we would add 2 ml of
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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the enzyme was reasonably active and starch hydrolysis was occurring. And lastly‚ the last column all enzymes were denatured. The control in the experiment was the first row‚ or 0 minutes‚ because the Starch was not mixed in with Amylase at that point proving that starch turns into a black complex and comparisons were
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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