Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix “chroma‚” which suggests “color‚” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment‚ chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate and identify amino acids‚ the building blocks of proteins
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BRONSTED-LOWRY ACIDS AND BASES 1. The Bronsted-Lowry definition There are many definitions of acids and bases in existence‚ but the most useful one is the Bronsted-Lowry definition: An acid is a substance which can behave as a proton (re presented as a hydrogen ion‚ H+) donor. Any substance which contains hydrogen bonded to a more electronegative element can thus behave as an acid: HCl == H+ + Cl- H2SO4 == H+ + HSO4- A base is a substance which can behave as a proton acceptor. Any
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Amino Acids Glycine is the smallest of the amino acids. It is ambivalent‚ meaning that it can be inside or outside of the protein molecule. In aqueous solution at or near neutral pH‚ glycine will exist predominantly as the zwitterion. Alanine is a hydrophobic molecule. It is ambivalent‚ meaning that it can be inside or outside of the protein molecule. The α carbon of alanine is optically active; in proteins‚ only the L-isomer is found. Serine differs from alanine in that one of the methylenic
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the concentration of the acid solution changes the rate of the reaction between dilute hydrochloric acid and magnesium metal. • As the metal reacts with the acid‚ hydrogen gas is produced and the metal dissolves. To get an estimate of the rate of reaction‚ the time taken for a small piece of magnesium ribbon to dissolve (disappear) can be measured. This is a “clock reaction” and the reaction rate is proportional to 1/time taken SAFETY Dilute hydrochloric acid is harmful. Wear safety spectacles
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structures and properties of amino acids know the groups of amino acid What are amino acids? Amino acids are molecules that when combined with each other proteins. Amino acids contain a central tetrahedral carbon atom (α-carbon) amine group‚ carboxyl group‚ R-side chain The R-side chain determines the different amino acids There are 20 common amino acids Amino acids can join via peptide bonds Several amino acids occur only rarely in proteins Some amino acids are not found in proteins 3D
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Standardization of NaOH and Determination of Unknown Acid KEYWORDS: quantitative analysis‚ titration‚ buret‚ endpoint‚ standardization‚ half-equivalence point‚ calorimetric titration‚ potentiometric titration ABSTRACT: The concentration of sodium hydroxide was determined by colorimetric titration‚ and the identity of an unknown acid was determined by potentiometric titration. In the first titration‚ a strong acid standard‚ potassium hydrogen phthalate (KHP)‚ was used‚ to determine the concentration
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------------------------- 1 Depression/ (88163) 2 Fluorodeoxyglucose F18/ (16267) 3 Deoxyglucose/aa [Analogs & Derivatives] (2344) 4 2 or 3 (16702) 5 1 and 4 (36) 6 (fdg adj1 pet).ti‚ab. (11503) 7 flourodeoxyglucose.mp. (35) 8 6 or 7 (11519) 9 2 or 3 or 8 (19574) 10 1 and 9 (39) 11 limit 10 to (english language and humans) [Limit not valid in PsycINFO; records were retained] (36) 12 exp Major Depression/ (84102) 13 9 and 12 (25) 14
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Synthesis of Salicylic Acid Experimental Data: 1. Mass of methyl salicylate used: 0.232 g 2. Theoretical yield of salicylic acid: 0.211 g 3. Volume H2SO4 added‚ with units (drops or mL): 3mL 4. Mass of crude salicylic acid obtained: 0.250 g 5. Volume of water used as recrystallizing solvent: 2 mL 6. Mass of purified salicylic acid: 0.134 g 7. Percent yield of purified salicylic acid from reaction: 63.5% 8. Melting point of purified product: 158-160 oC 9. Name of NMR solvent used and
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Nitric acid is a highly reactive oxidizing agent used in making fertilizers‚ explosives‚ and rocket fuels‚ and in a wide variety of industrial metallurgical processes. It is also a component of acid rain. Its chemical formula is HNO3 and it has been known as “aqua fortis”‚ which means strong water‚ to alchemists. It is a transparent‚ colorless to yellowish‚ fuming corrosive liquid. Nitric acid is a strong acid and therefore it completely dissociates in water. It has a gravity of 1.41 and the concentration
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Depression is a serious mental health concern that will touch most people’s lives at some point in their lifetime (either directly or through someone close they know). The suffering endured by people with depression and the lives lost to suicide attest to the great burden of this disorder on individuals‚ families‚ and society. Improved recognition‚ treatment‚ and prevention of depression are critical public health priorities. Organizations such as the National Institute of Mental Health (NIMH)‚ one
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