University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
INTRODUCTION During collisions involving two bodies‚ equal and opposite forces are set up between them. These impact forces influence the subsequent motion of the bodies. Momentum of the system (consisting of both bodies) is preserved if both bodies are free to move in space. This is because there is no external forces act on the system. The forces acting between the bodies during the small interval of time when they are in contact cause changes in the velocities of each separate body. An
Free Kinetic energy Classical mechanics Energy
Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
Free Cell Microscope Cell wall
Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
Premium Light Photosynthesis Color
Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
Premium Antibody Immune system Chemistry
Background examination for the Dominant Thumb Experiment: • Upon reading the literature that the researcher found by googling‚ "Thumb Dominance‚" they discovered that almost every human being has a dominant thumb whether it be the right thumb or the left thumb on top after naturally clasping their hands together. However‚ if the individual tries to force their clasped hands to let the other thumb become dominant it ’s most often described as feeling awkward or abnormal. In 1999‚ a scientist by the
Premium Finger Human Primate
Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
Premium Flatworm Digestion Petri dish
Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
Premium Plasmid Escherichia coli Agar plate
A World Without Friction (603 Words)Although friction may seem like a minor issue‚ its absence would chance life as we know it. There would be many negative effects of this like not being able to gain traction on any surface and your ipods earplugs always falling out. Also‚ there would be many positive effects‚ such as eliminating the need for coolant in engines and lubricant in machines. Moreover‚ the absence of friction would be both bad and good. Without friction‚ many obvious things which we
Premium Plate tectonics Superconductivity Friction
Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
Premium Food coloring Color E number