Unknown Microorganism: #5 Report Callie Schalk Microbiology Professor Quave November 24‚ 2015 The unknown microorganism #5 is Pseudomonas aeruginosa. It can survive in a variety of environments and at temperatures found in clinical settings (Murray et al‚ 1999). Clinical setting is referring to temperatures such as those in the hospital environment. P. aeruginosa can also survive in aqueous environments. Pseudomonas aeruginosa is found at several different sources. Nosocomial
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Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was
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the density of water and an unknown liquid. Density is defined as the mass of a substance divided by its volume. It is an intrinsive property of matter and is used to specifically characterize substances. III. Procedure: 1.We massed an empty 10mL graduated cylinder to the nearest .01g. 2.Then‚ we filled the graduated cylinder with 4.0-5.0 mL of distilled water‚ 3.massed it to the nearest 0.1 ml and 4. recorded the data. Then to determine the density of the unknown liquid; we repeated the steps
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One of the most well known area visited of Arlington National Cemetery is the Tomb of the Unknowns. Many of the soldiers buried at the Tomb of the Unknowns fought in World War One. Additionally‚ millions of soldiers had died during the first World War and many American soldiers were brought home from the European battlefields and many others’ were never identified. On October 21‚ 1921‚ four coffins were dug up from the four American military cemeteries‚ which were located in France. Corporal
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized
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Tiffany Ward Scheme For Unknown 2A/B Possible Ions: Cations: Na+‚ K+‚ NH4+ Anions: OH-‚ Cl-‚ NO3-‚ SO42-‚ HSO4- 1.Describe Sample Phase‚ color‚ odor‚ crystal shape‚ amorphous‚ gel-like‚ needle-like‚ powdery‚ etc. 2.Tests on original sample (i) Flame test bright orange Na+ purple K+ no color NH4+ (ii) NH4+ test Place a small portion of original unknown sample in a test tube or spot plate‚ then add a few drops of 1M NaOH solution. At the same time suspend a piece of red
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Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
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