Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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Advertising & Sales Promotion “Parle-G” Submitted By: Varun Gupta : PG20095696 Vikram Sharma : PG20095098 Index S. No. Content Page Number 1 Company and Brand Details 1 2 Agency Details 3 3 Consumer and Brand Positioning 4 4 Consumer Response Model—Stage 5 5 Source Message and Channel Factors 6 6 Objectives for the Brand 7 7 Consumer feelings (FCB Grid) and appeals recom-mended 8 8 Designing Communication 9 9 IMC—Recommended Mix for Parle-G 10 10 Sales Promotions—Recommended options 11 11 References
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In a case regarding the shape of a flashlight; Mag Instruments ‚ the European Court of Justice (ECJ) ruled that the relevant public’s perception of 3D trademarks was certainly different from its perception of word and verbal trademarks. As indicated by the ECJ‚ if a realistic or printed component is inadequate with regards to‚ general society is not used to inferring the source of the item from its shape or bundling. On account of a 3D trademark‚ deciding uniqueness could be more troublesome than
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Figure -1 shows a relatively simple supply chain‚ which links a company with its suppliers (on the left) and its distributors and customers (on the right). The upper part of the figure shows a generic supply chain; the lower part shows the chain of a toy manufacturer The supply chain is linear and it involves three basic parts Downstream supply chain Internal supply chain Upstream supply chain Tanzila Tahrin ID No: 114911 Types of Supply Chain Integrated make-to-stock The integrated make-to-stock
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2012 2011 2010 2009 2008 200 Net Sales $ 83‚680 $81‚104 $77‚567 $ 75‚295 $ 77‚714 $ 71‚095 Gross Profit 41‚289 41‚245 40‚525 37‚644 39‚534 36‚607 Operating Income 13‚292 15‚495 15‚732 15‚188 15‚743 14‚236 Net Earnings from Continuing Operations 9‚317 11‚698 10‚851 10‚645 11‚224 9‚562 Net Earnings from Discontinued Operations 1‚587 229 1‚ 995 2 ‚877 9 30 847 Net Earnings attributable to Procter & Gamble 10‚756 11‚797 12‚736 13‚436 12‚075 10‚340 Operations 11.1% 14.4% 14.0% 14.1%
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To what extent did opposition to the policy of appeasing Germany increase in Britain in the years 1936-1939? The policy of appeasement had reached its heights by the period between 1936 and 1939. It was felt by many to be the best policy at the time‚ as it allowed Britain to buy herself some valuable time in order to delay the inevitable war. Opposition during 1936‚ when appeasement was first seen as really taking the forefront of foreign policy‚ was small and weak. However it was by 1939 that
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Effectiveness of Eggplant (Solanum melongena) Extract and Gabi Corms (Colocasia esculenta) as Mosquito (Aedis aegypti) Repellent A Research Proposal presented to the faculty of the Science and Technology Department In partial fulfillment As a requirement in Research II Paul Brian Marcuelo Grade 8-Pollux Proponent Ma’am Sharon Villagracia Research Adviser Negros Occidental High School Bacolod City‚ Negros Occidental December‚ 2014 INTRODUCTION Beyond the nuisance factor‚ mosquitoes are
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G.G. Toys – Nele Rieve – E01487695 – 10/14/2014 G.G. Toys is a leading manufacturer of high-‐quality dolls located in the US. The company is popular for its “Geoffrey dolls” but‚ due to rising product costs‚ has included customized dolls and cradles in its product mix. Two plants are used for the
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P&G Korea Case Study The main issue of the P&G Korea case is centered around the question of market share. P&G and Unilever are the two major market shareholders in the Korean detergent industry holding 80-85% of the total market share. The remaining 15-20% of the market is held by low-priced local Korean brands. There are no new markets either company can tap for further market share since most Korean households already use laundry detergent‚ making the market saturated. Other than peripheral
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3.2 Targeting for launching the traditional toy: Targeting is basically selecting an aimed segment after evaluating various market segments. With the completion of market segmentation‚ it is necessary to do the targeting of the market after carefully evaluating each segments ( Armstrong & Kotle‚ 2014). The company now requires to decide‚ which market segment needs to be concentrated‚ in order to reach the company’s goals and objectives. The toy company has three different strategic approaches
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