Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Investigation Investigate the amount of heat evolved when magnesium reacts with dilute acids. Planning What I am going to do I am going to find out how much heat is given out when magnesium reacts with a variety of dilute acids. In order to make comparisons between the acids‚ I shall use my results to work out how much heat would be given out if 1 mole of magnesium reacted with an excess of each acid. The acids I shall use are hydrochloric acid‚ sulphuric acid‚ nitric acid and ethanoic acid
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Title: PROP0332 Evaluation of the Gas Law Constant Abstract: The result of the change in volume was approximately 22 CC or 0.00084 mol. This translates into the average for the R constant being 83.8L*atm/K*mol. The four determinations ensured that the results were accurate because more than one trial helps somewhat prevent error. Approximately 0.20g of the Mg ribbon was used for these determinations. Introduction: 1. Theory If the temperature of a gas sample was held constant‚ its volume
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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reproductive. Orientation is when the organism is placed in their beneficial environment consisting of two behaviors‚ taxis (movement directly towards or away something) and kinesis (random movement). Reproductive is detected using drosophila in this lab‚ it consists of finding courting and mating with another of the species Agnostic behavior is found in a situation where the animal feels threated by another‚ leading to the organism looking bigger or more threatening to the opponent. Within the Agnostic
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is involved with the cannon being fired then the force of gravity would cause the cannon to start to slow down‚ wouldn’t go as far and the direction or trajectory of the cannon ball would be a curved line going down. Purpose: The purpose of this lab is to give the student a better understanding about projectile motion and to understand Newtons theories and laws of motion. We will be firing the plastic ball from the projectile launcher three times from eight different angles from the ground &
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Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and the other consists of two liquids
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extinguished within 7 second . When the glowing splinter is pulled out ‚ it rekindled . Discussion The equation involved in this experiment is decomposition of hydrogen peroxide . H2O2 ( liquid ) → H2O ( liquid ) + O2 ( gas )
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