Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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Performed on: Wednesday‚ May 8th‚ 2013 | | | 5/15/2013 | | Introduction: The goal of this experiment was to identify an unknown analgesic drug through the use of thin-layer chromatography. The drugs used were‚ Unknown #1‚ caffeine‚ aspirin‚ and acetaminophen. Thin-Layer Chromatography is a technique for identifying substances using a mobile phase (developing solvent) that draws up through a stationary phase (TLC plate) and marks spots of differing distances for differing substances
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Experiment # 4 Paper Chromatography 1. Why is the chromatogram developed in an essentially closed system? - The chromatogram is developed in a closed system in order to prevent the solvent to evaporate. Most solvents used in the chromatograph are toxic and flammable. It is also put in a close system to reduce the chance of outside factors affect the chromatograph. 2. What is the main advantage of 2-dimensional paper chromatography over a 1-dimensional one? - The
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Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and the other consists of two liquids
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COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana‚ Mariah Ericka M. Patawaran‚ Princess Juneire M. Peligro‚ Francisco Q. Pua III‚ Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography‚ as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
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Lab #1 Extraction and Thin Layer Chromatography Kaya Gaudet 6784928 January 9‚ 2013 Line Structure References BenzophenoneBiphenylBenzoic Acid Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the impurities
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Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix “chroma‚” which suggests “color‚” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment‚ chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate and identify amino acids‚ the building blocks of proteins
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effective activated carbon‚ rice hull was used in studies that include the aforementioned sample. Because of the problems in the environment and the availability of the samples that can solve it‚ this encouraged the researchers to invent an exhaust gas filter with coconut fibers and granular activated carbon from rice hull. Statement
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silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin‚ and the thin layer chromatography for analysis.It was concluded that the renention factor(Rf) of the 5‚10‚15‚20- Tetraphenylporphyrin with a percent yield of 61% Introduction: In this experiment 5‚10‚15‚20‚ tetraphenylporphyrin
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