for 10 minutes and the supernatant was transferred to a new tube. Isopropanol was then added to precipitate the plasmid DNA and centrifuged into a pellet‚ dried‚ and then resuspended with distilled water. This plasmid DNA was then run on an agarose gel to view the stands of
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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It was hypothesized that if the animal was larger‚ then it would have larger hemoglobin. It was thought that larger mammals would need more oxygen-rich hemoglobin to support healthy function. This lab did not support this hypothesis. The results in table 1 displayed that the hemoglobin of all mammalian samples traveled about the same distance. This trend can be seen in graph 1 as the mammalian hemoglobin samples migrated the same distance. Because the proteins traveled around the same distance it
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
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Conclusion In part A of this experiment‚ we transformed the bacteria into an antibiotic resistant form by inserting a plasmid into it. We used heat shock in order to make the bacteria capable to uptake a plasmid in the presence of calcium ions that help disrupt the cell membrane (heat shock is the combination of altering hot and cold). When they are capable of accepting plasmids‚ the bacteria are incubated with plasmids that carry the resistance to a particular antibiotic‚ in this case ampicilin
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City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
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Oscillators & Oscilloscope Morgan Dennis @02514008 Partners: Brittany Smith‚ Michelle Trang‚ James Anderson‚ Madison Shaw OBJECTIVE: We are going to study the features and operation of the oscilloscope. We will use the instrument to measure the frequency and amplitude for various sources. Ultimately‚ the oscilloscope will display the Lissajous figures. APPARATUS: The major apparatus used include; Oscilloscope‚ two audio oscillators‚ battery and connectors. THEORY: The oscilloscope
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Introduction Memory is defined as the faculty by which the mind stores and remembers information(Eysenck & keane‚ 2010). Atkinson and Shiffrin (1968) (Cowan‚ Rouder‚ & Stadler‚ 2000)‚ came up with a model of sensory memory which stated not everything we perceive we process. Craik and Tulving (1975) developed an opposing theory in which they proposed the levels of processing theory‚ stating its not the processing but the type of quality of the processing that is performed‚ that determines the retention
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