sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through the gel and the sequencer reads the bases
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Purpose The purpose of this experiment was to become familiar with the electrocardiograph to help us determine the electrical activity of the heart. As well as‚ to observe how exercise and different body positions(conditions) can affect the activity of the heart. Introduction Trained professionals can look at the EKG tracing and determine if the heart is normal or if the heart is abnormal. “An ECG records the electrical activity of the heart. The heart produces tiny electrical impulses which spread
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Abstract: The purpose of this lab experiment was to discover genetically modified organisms. We analyzed our results and found that our sample was GMO positive. These results were attained through polymerase chain reaction and agarose gel electrophoresis. We came to the conclusion that the presence of a band in lane 4 confirms that there are indeed genetically modified organisms. Introduction: Overall‚ the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in order
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SCIE211 Phase 2 Lab Report Title: Speciation Instructions: You will need to write a 1-page lab report using the scientific method to answer the following question: • What would happen if a species within a population were suddenly split into 2 groups by an earthquake that creates a physical barrier like a canyon? When your lab report is complete‚ post it in Submitted Assignment files. Part I: Use the animated time progression of speciation to help you write up your lab report. Part II: Write
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into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes and cuts the palindromic base pair sequence GATTC while Pstl recognizes and cuts the palindromic base pair sequence CTGCAG. Agarose gel electrophoreses
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FST 4822 LABORATORY FOR CHEMISTRY AND TECHNOLOGY OF PLANT AND ANIMAL PRODUCTS Group : | 8 | Title : | Processing of yoghurt | Date of experiment : | 30 September 2009 | Lecturers : | Profesor Madya Dr. Azis Ariffin and Dr. Anis Shobirin Meor Hussin | Group members: | Matric Number | Nor Haafiz Bin Idris | 140532 | Wan Nur Mazurawan Mansor | 141377 | Nur Ain Salmi Abdul Hamid
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The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
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Introduction The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast‚ EAY 235 and EAY 431‚ which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion‚ which caused EAY 431 to be a deficient
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Physics Lab Report Format General Remarks: Writing a lab report is the only way your TA will know what you have done during the lab and how well you have understood the process and the results. Part of your lab experience should be learning how to organize and present your work in a scientific way. There is no framework that can be used as a “one size fits all”‚ therefore this sample lab report should only be used as an example. Any lab report should have the following features:
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