Hassuneh. Report subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed
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2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is placed
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8.8 and stacking gel‚ prepared using
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Introduction During the course of this lab‚ we explored whether or not certain processed foods contained Genetically Modified Organisms. Genetically modified food is an important subject in the world today. Ever since Flavr Savr came out with their tomatoes grown from genetically modified seeds in 1994 genetically modified foods has become ever more popular amongst distributors of produce (Mestel‚ 2013). The world’s population has grown by around one billion in the last decade (US Bureau of the
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate
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Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
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