LISMA RUHILA BT ALIAS Group: AS201 5A Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER 2012 OBJECTIVE * To study measure
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Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled into a gel. Agarose gel is most commonly
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Title: Grip Strength and Muscle Fatigue I. Purpose/Hypothesis: In this lab the subject will grip on a scale that measures the amount of force being put on it. We will observe the change in hand strength during continuous grip over time. Then we will also measure hand strength during rapid‚ repetitive gripping. I believe that hand strength will weaken during a continuous grip more than if you were to keep a repetitive grip. II. Procedure: 1. Connect the Hand Dynamomenter to LabQuest and select
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Title: Principles and Practice of Agarose Gel Electrophorsis Objectives: To detect the size ‚ shape and charge of the each dye solution. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the intire lab class the equation had to be multiplyed by five. The contents of this equation were added to the 250ml flask and swirled to evenly distrubute it contents
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Bernardine Date Due: 02/08/2013 PURISIMA‚ Dio Mark Angelo Date Submitted: 02/08/2013 Experiment No. 9 AGAROSE GEL ELECTROPHORESIS OF DNA Abstract _____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform
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Courtney Jablonowski Dr. Peterson Lab Report 1 June 17‚ 2012 Results The tooth formula is a shorthand technique that biologist use to keep track of the teeth on a particular jaw. The formula consisted of two lines‚ top jaw and bottom jaw‚ which were used to count how many incisors‚ canines‚ premolars‚ and molar teeth a species had. Species A‚ Odocoileus virginiaus (white-tailed deer)‚ had a tooth formula of 0033/3133. On the top jaw‚ the deer had zero incisor and canine teeth but have
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How does the process of gel electrophoresis separate DNA fragments? It uses an electric current to separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has an
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Transpiration Lab Write-up. Introduction Transpiration is the evaporation of water from plants. It’s not simply a hazard of plant life but it’s the engine that pulls water from the roots to cool the leaf and supply photosynthesis. The concentration of water vapor in the atmosphere is lower than that in the leaf. Because of this difference‚ water vapor diffuses from the spaces of the leaf‚ through the stomata in the epidermis. Stomata are in the lower epidermis; the lower surface receives less
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How to Write a Lab Report Lab reports are formal papers that describe and analyze lab experiments. They follow a very specific format. If you study this paper carefully and follow its directions‚ you will find that these papers are not at all difficult and can have a positive impact on your Biology grade. Paper formatting: The paper should be typed and double spaced. Use a standard 12 point font (do not use cute fonts or colored paper!). Except for the title and abstract – each section
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Bacteria Lab Write-Up Jianna Liu P8 Purpose: The purpose of this lab is to find out which substance‚ alcohol‚ antibacterial soap‚ water‚ or hydrogen peroxide‚ is the best at preventing the growth of bacteria. Hypothesis: Antibacterial soap will be the best because soap is a substance that people use to wash their hands because it takes away the germs. Even the name‚ antibacterial soap‚ suggests that it fights off bacteria. Hydrogen peroxide is second because it is used
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