Conclusion In part A of this experiment‚ we transformed the bacteria into an antibiotic resistant form by inserting a plasmid into it. We used heat shock in order to make the bacteria capable to uptake a plasmid in the presence of calcium ions that help disrupt the cell membrane (heat shock is the combination of altering hot and cold). When they are capable of accepting plasmids‚ the bacteria are incubated with plasmids that carry the resistance to a particular antibiotic‚ in this case ampicilin
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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some generalizations about the poet’s work and this poem’s relation to the poet’s work as a whole‚ as well as a summary of what happens in the poem 2. for a passage from a play or novel‚ a brief summary of the action leading up to the passage‚ as well as a summary of what occurs in the passage itself B. Identify the speaker/narrator 1. for a poem‚ basic evidence of who the speaker is from within the poem 2. for a passage from a play‚ the mood or state
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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II. Structure of a thesis proposal Your thesis proposal should have the following elements in this order. Title page Abstract Table of contents Introduction Thesis statement Approach/methods Preliminary results and discussion Work plan including time table Implications of research List of references The structure is very similar to that of a thesis or a scientific paper. You will be able to use a large fraction of the material of the thesis proposal in your final thesis. Title page
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carbohydrates. Procedure Preparing the lab supplement: First‚ canned spinach supplied by the organic chemistry laboratory in room
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Phase 1 Lab Report Title: Human Impacts on the Sustainability of Groundwater Instructions: You will need to write a 1-page lab report using the scientific method to answer the following question: If current human development does not change‚ will groundwater sustainability be affected? When your lab report is complete – submit it in the classroom. Part I: Using the time progression of industrialization and human development‚ fill in the data table below to help you write up your lab report
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BIOMAGNIFICATION LAB REPORT AIM The aim of this lab is to model bioaccumulation and biomagnification through a food chain. MATERIALS 100 M&M’s Paper towel to lay M&M’s on 20 small cups labelled “zooplankton” 5 medium cups labelled “minnow” 2 larger cups - one labelled “eel #1”‚ and another labelled “eel #2” 1 bowl labelled “osprey” PROCEDURE The pile of M&M’s represents the phytoplankton population in a lake. The printed “M” on the candy represents the amount of DDT (in ppm)
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Solve the Murder Koreen Clarke October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette
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