Josh P. AP Biology 12-10-13 Lab Writeup The Effect of Transformation of pGLO in Bacteria Background/Objectives/Hypothesis: Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab‚ bacteria was transformed by inserting DNA for Green Fluorescent Proteins. The DNA for these proteins were taken from bioluminescent jellyfish Aequorea
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What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
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protein electrophoresis. Protein electrophoresis involves the movement of proteins within an electric field with mobility being dependent on factors such as the size and shape (secondary and tertiary structure)‚ as well as the charge of the protein (due to primary structure). Other factors that can affect the mobility are electric-field strength‚ matrix pH‚ and ionic buffer strength of the electric field. Because there are so many factors involved in analyzing proteins during electrophoresis‚ it is
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Photosynthesis Lab Kozlowski Kendal Schweiss Hour 6 Introduction: Photosynthesis is carbohydrate production using light and chlorophyll. It is a process when green plants and other organisms turn carbon dioxide and water into carbohydrates and oxygen‚ using light energy trapped by chlorophyll. The rate of photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. 2H2O + CO2 + light -> carbohydrate (CH2O) + O2 + H2O There is a spongy mesophyll
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Chemistry Lab Write-Up Title: Paper Chromatography of Powdered Beverage Mix Purpose/Objective: The purpose is to be able to use liquid chromatography to separate dyes in a powdered beverage mix‚ calculate the Rf values for each dye‚ and rank the polarity of each dye present. Hypothesis: If we are to use the liquid chromatography to investigate the different dyes‚ then I think the different dyes will come up having different polarities and Rf values. Procedure/Method: Draw a line across
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Egg-speriment Write-up Part 1- Purpose: Why did we do the experiment? The purpose of this experiment was to understand how osmosis works and that the cell membrane is selectively permeable. Osmosis is the diffusion of water molecules through a selectively permeable membrane. Many cells cannot function without adequate water‚ so cells depend on osmosis. When a cell is selectively permeable‚ it means some substances can pass through while others can’t. Many examples of these were shown throughout
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waterBoiling chipProcedureA Separation of a Sodium Chloride and Charcoal Mixture#1 Place 4 g of the salt/charcoal mixture in a 100 mL beaker and add about 15 mL of distilled water. Stir the mixture for about two minutes to allow the salt to dissolve. #2 Set up a filter funnel with filter paper on a filter funnel stand. Filter the mixture and collect the filtrate in a 100 mL beaker as shown in Figure 1.1. #3 Wash the solid with a further 5 mL of water but do not add this to the filtrate. Note and record
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March 18‚ 2013 Spirometry Lab Write-Up A. Subject History The subject is a twenty eight year old female. She stands five feet eight inches tall and weighs approximately one hundred fifty pounds. The subject is a non-smoker and although she has never had any respiratory issues‚ asthma does tend to run in her family on the maternal side. The subject does however admit to feeling out of breathe easily when doing intense exercise for an extended period of time. B. Effect of CO2 on Breathing
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EXPERIMENT WRITE-UP INTRODUCTION: Information entering the memory can take on different forms‚ e.g. images‚ sounds or meaning. A way in which information is processed is through encoding‚ which is when information enters the sensory memory and is changed into a form that the memory can store it in. Information can be encoded as visual‚ acoustic or semantic. Evidence suggests that the primary encoding method for information to be stored in the short term memory (STM) is through acoustic coding
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Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markers Abstract This
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