protein electrophoresis. Protein electrophoresis involves the movement of proteins within an electric field with mobility being dependent on factors such as the size and shape (secondary and tertiary structure)‚ as well as the charge of the protein (due to primary structure). Other factors that can affect the mobility are electric-field strength‚ matrix pH‚ and ionic buffer strength of the electric field. Because there are so many factors involved in analyzing proteins during electrophoresis‚ it is
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Biocompatible nanomaterials are revolutionizing many aspects of preventive and therapeutic healthcare‚ which are already playing important roles in developing new medical devices‚ prostheses‚ bioassay technologies‚ tissue repair and replacement technologies‚ drug delivery systems‚ and diagnostic techniques. In recent years‚ much attention has been focused on the synthesis and applications of biocompatible functional nanomaterials‚ owing to their promising potential and practical applications
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separate mixtures of naturally occurring compounds isolated from plants and other living organisms. Performing column chromatography involves packing a column‚ which is a glass cylinder‚ with the stationary phase. The stationary phase is typically silica “gel” or powdered alumina‚ depending on the types of compounds you wish to separate. The mixture is placed on top of the packing material and a steady‚ continuous flow of eluting solvent is passed through the column. Although in the TLC experiment only one
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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William son Eth er Synth esis Introduction In this reaction‚ an alcohol is deprotonated to form a good nucleophile‚ which then attacks the electrophile methyl iodide to form an ether. Tetrabutylammonium bromide‚ a phase transfer catalyst‚ is used to carry ions back and forth between the organic phase and the aqueous phase. OH O NaOH + NaI + H2O CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating
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Dr.Tawfeq al.Qaimare Rifqa Shawer Lab report 5 21-3-2013 Adsorption Chromatography Introduction: The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube‚ it’s a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column‚ giving this region a nearly dark blue color. However‚ when
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The chemistry of hairspray What is hairspray: introduction Hair spray (or hair lacquer) is a common household aqueous solution that is used to keep hair stiff or in a certain style. Weaker than hair gel‚ hair wax‚ or glue‚ it is sprayed to hold styles for a short period of time. Using a pump or aerosol spray nozzle it sprays evenly over the hair. Hairspray was first developed and manufactured in 1948 by Chase Products Company‚ based in Broadview‚ Illinois.Its active ingredient is a suitable
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to separate mixtures into their compounds. It’s based on a mobile phase and a stationary phase. The mobile phase moves up the thin layer paper by taking the compounds with it‚ till it’s absorbed and stops moving. [1] The paper chromatography (silca gel) is a very uniform absorbent paper. Different components travel at different rates. Depending on the affinity each component has‚ it will move faster or slower up the TLC. To visualize the location of the different spots‚ an ultraviolet lam is necessary
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iIsolation of Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately
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Chapter I. The Problem and it’s Background Introduction Aloe Vera is a species of succulent plant that probably originated in northern Africa. The species is frequently cited as being used in herbal medicine since the beginning of the first century AD. Extracts from A. Vera are widely used in the cosmetics and alternative medicine industries‚ being marketed as variously having rejuvenating‚ healing or soothing properties. Aloe Vera is one of the only known natural vegetarian sources of Vitamin
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