Isolation of Recombinant Taq Polymerase for PCR Isolation of Recombinant Escherichia coli IPTG induced Taq polymerase and characterization through polymerase chain reactions‚ Western Blotting and gel electrophoresis * Braeden Cowbrough1‚ Michael Atkins2‚ Christopher Bonner3 From the Faculty of Biochemistry Lab 3006 B Carleton University‚ Ottawa‚ ON K1S 5B6 *Running title: Isolation of Recombinant Taq Polymerase for PCR To whom correspondence should be addressed: Braeden Cowbrough‚ Faculty of Biochemistry
Premium Polymerase chain reaction Molecular biology
of long-term ketamine administration on rat bladder protein levels: A proteomic investigation using two-dimensional difference gel electrophoresis system Abbreviations & Acronyms 2D-DIGE = two-dimensional difference gel electrophoresis CHAPS = 3-[(3Cholamidopropyl) dimethylammonio] propanesulfonate Cy = cyanine dye DIGE = difference gel electrophoresis DTT = DL-Dithiothreitol GAPDH = glyceraldehyde 3-phosphate dehydrogenase HK = high dose of ketamine IC = interstitial cystitis
Premium Urinary bladder
-Tique Corporation must decide if and how the company will introduce an aerosol can package for its Soft and Silky Shaving Gel‚ including a determination of product size (5.5 or 10 oz.) and location (personal care or toiletries). Furthermore‚ the manager needs to decide if a test market is necessary to evaluate the new packaging. Strategic Issues Product Soft and Silky Shaving Gel (SSSG)‚ a women’s shaving product that contains moisturizers‚ is currently packaged in a 5.5 oz. plastic tube and is located
Premium Marketing Product management Revenue
------------------------------------------------- TNS SHOWER GEL CASE POSITIONING MAP BRAND 7.3 BRAND 8.1 BRAND 7.4 BRAND 6.2 BRAND 3.3 BRAND 4.1 BRAND 7.1 BRAND 3.2 BRAND 5.1 BRAND 2.2 BRAND 3.1 BRAND 4.2 BRAND 1.1 BRAND 7.2 BRAND 2.3 BRAND 2.1 BRAND 1.2 BRAND 6.1 Shelf-Space 0.2 Private Label 1.2 0.8 0.0 0.6 1.4 1.8 1.0 Price/Volume 0.4 1.6 ≥0‚3l <0‚3l and ≥ 0‚25l Unit Size <0‚25l Economy Packs Middle of the market Luxury Brands and Sports Gels In the above positioning map
Premium UCI race classifications Tour de Georgia Marketing
INTERNSHIP REPORT ON Hair Care Survey on Parlors & Salons in Dhaka Guided by Ms. Farhana Nur Malik Lecturer BRAC Business School Ms. Ankan Siddiquee Brand Activation Manager Unilever Bangladesh By Kamrun Nahar ID-08104011 BRAC Business School Brac University Date of Submission: 19 December 2011 LETTER OF TRANSMITTAL 19 December‚ 2011 Ms. Farhana Nur Malik Lecturer BRAC Business School BRAC University Subject: Submission of internship report Dear Madam‚ I would like to take this opportunity
Premium Unilever Hairdressing Hairstyle
Anonymous Dr. Mason English 1102 14 February 2014 Bench Fix Advertisement In many ways‚ people all over the world get sucked into buying hairstyling products that either benefit them or empties their pockets. All these commercials and advertisements on hair products consist of these small elements or components that help persuade the audience to either take action through their claim or support their claim. Bench Fix introduces their hairstyling product with several of these techniques and
Premium Hairstyle Advertising A Great Way to Care
Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
Premium Thin layer chromatography Chromatography Analytical chemistry
The Chromatography of Food Dyes and determination Of the Dyes Present in M&M Candies Procedure To complete this lab‚ I first mixed the solvent solution consisting of the proper ratio of water‚ salt‚ and isopropyl alcohol in a Pyrex measuring cup. I then prepared my chromatography papers for 2 trials by drawing‚ with a pencil‚ the appropriate lines and labels. Using a toothpick‚ I added the color to the paper‚ and then repeated until the color was strong. I repeated this step several more
Premium Chemistry Chromatography Analytical chemistry
A Most Mysterious Material: The Aerogel In his book‚ “Stuff Matters”‚ Miodownik discusses‚ through personal narratives‚ the many facets of material science and their applications in real world instances. Out of all of the different types of materials he encounters‚ perhaps the most exotic were aerogels. In this chapter‚ “Marvelous‚” Miodownik begins in a top secret U.S. government laboratory where he first sets his eyes on this mysterious material. At first‚ Miodownik is denied a chance to observe
Premium Materials science Light Wavelength
Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
Premium Chromatography Analytical chemistry Silica gel