Purpose In this lab‚ we used PCR and gel electrophoresis to identify genetically modified food. Introduction A genetically modified organism is an organism whose DNA or genetic makeup has been modified to code for certain desirable traits("Genetically Modified Foods"). Common genetically modified plants include corn and soy‚ and common genetically modified animals are fish. Many genetically modified plants are coded to resist bugs‚ grow faster‚ and produce bigger fruit‚ while most GMO animals
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Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markers Abstract This
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Bio 1 Lab: Electrophoresis and DNA fingerprinting Jani Lynette Hagen October 31‚2014 U74644799 Electrophoresis is a technique which uses an electric field to separate molecules‚ allowing for identification and characterization of the molecules. It is commonly used to separate nucleic acids and protein molecules of various sizes. To prepare the gel for electrophoresis the amount of agrose needed must be calculated. For a 0.8 percent gel 0.8 grams of agrose is necessary per 100 ml of buffer. The DNA
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards the positive charge. The results obtain from the gel electrophoresis able to concluded the which suspect have been at the crime scene by analyzing against the
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Lipid and protein composition varies in tissue types. Results: When comparing liver‚ muscle‚ and adipose tissue‚ adipose tissue had the highest percent lipid and liver tissue had the highest percent protein. Conclusion: Based on analysis of the gels‚ the liver appeared to have the most variety in proteins when comparing liver‚ muscle‚ and adipose tissue. Significance: In order to determine the composition of a tissue‚ specific macromolecules can be extracted‚ quantified‚ and analyzed. ABSTRACT
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The second part of the experiment deals with Gel Electrophoresis. The samples are loaded into wells on an 1% agarose slab and subjected to electrical currents both positive and negative. Our current target here is DNA‚ therefore since nucleic acid as a negative charge‚ the bands will migrate toward the positive cathode. This process of migration is called sieving and smaller strands move faster than longer strands due to their ease in going through the gel. The objectives of the experiment include:
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experienced (2). Gel electrophoresis is a technique used to separate mixtures of dna/ proteins based on what type of gel is used. Agarose gels are typically used for separating DNA and RNA. While polyacrylamide is typically used to separate out proteins and tiny amounts of nucleic acids. Gel electrophoresis of macromolecules separates molecules based on size shape length and charge. Gel electrophoresis is facilitated by the
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sealed with Parafirm and was placed in the refrigerator for next day lab. Other group was prepared other buffers that needed for this experiment. They are 0.5M Tris pH 6.8‚ 1x-running buffer‚ and transfer buffer. Day 2: Buffer preparation and run the gel and the samples First‚ the buffer was prepared by using the formula as follows:
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specific code determines the number of times this set of scissors will snip and the number and size of DNA pieces that will be left behind. These pieces can then be separated and compared using the process of gel electrophoresis. As these fragments move‚ their varying lengths propel them through the gel at different speeds. Scientists can use these RFLPs‚ Restriction Fragment Length Polymorphisms‚ a set of DNA puzzle pieces unique to only you‚ to create a pattern called a DNA fingerprint. Similar to the
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