This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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using gel electrophoresis‚ and then estimating their fragment sizes. The size and number of fragments help to determine information about the structures of the original DNA pieces from which they were cut. To determine the size of an unknown DNA fragment that was inserted into a plasmid‚ gel electrophoresis is used. This technique is used in crime scene investigations. Gel electrophoresis separate charged molecules‚ including nucleic and amino acids‚ by how fast they migrate through the gel under
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Recombinant DNA in E. coli Expresses Devastator Enzymes to Clean Oil Spills Dr. Land University of the Pacific‚ Stockton Abstract The bacteria Sulfolobus Oileatacus has an enzyme called the Devastator‚ DevA‚ that helps metabolize crude oil. Since the bacterium is a thermophile‚ it won’t survive at normal temperatures and cannot be used to clean up oil spills. The devA gene was cloned through the polymerase chain reaction and was confirmed to be present using a restriction enzyme digest;
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the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis. First‚ bacterial transformation to E. Coli DH5 was performed on our unknown plasmid
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3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method used for the amplification of DNA
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primer attached to a DNA strand. DNA Gel Electrophoresis Click on the following link and perform the gel electrophoresis experiment: http://learn.genetics.utah.edu/content/labs/gel/ 1. What is the function of gel electrophoresis? Way to sort and measure DNA strands according to length. This technique is also useful for separating other types of molecules‚ like proteins. 2. Where are DNA samples placed in the
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curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of base pairs of the fragments in the non marker samples using the standard curve created with the marker sample. It was hypothesized that if three samples of DNA are mixed with different restriction enzymes and 1 sample is not mixed with a restriction enzyme and agarose gel electrophoresis is performed on all 4 samples‚ then the patterns shown in the gel will be different and the sample with with no
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