Teacher Guide: DNA Fingerprint Analysis Learning Objectives Students will … Compare the DNA fingerprints of several individuals. Identify individuals that could be identical twins. Use DNA fingerprints to identify codons that control certain traits. Predict an individual’s phenotype using the individual’s DNA fingerprint. Vocabulary codon‚ DNA‚ DNA fingerprint‚ genotype‚ identical twins‚ nitrogenous base‚ phenotype‚ trait Lesson Overview With the exception of identical twins‚ no two organisms
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Nervous System and Sensory Organs • dorsal roots and ventral roots - connect Spinal nerves to the spinal cord • medulla -responsible for many involuntary functions such as heartbeat and breathing‚ primary communication pathway between the spinal cord and the rest of the brain‚ • cerebellum - receives input from multiple sensory receptor types and uses this information in coordination of complex body movements • pons- communication between lower and higher brain regions • midbrain- processes
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Sample Methodologies for DNA Extraction and PCR for gender identification of Gallus gallus domesticus INTRODUCTION: DNA extraction and PCR have been used in numerous research projects‚ current research commonly utilise such techniques for the basis of their study and as economic cost of the proceduredecreases it will become even more prevalent in industrial and commercial settings. A variety of methods collection can be employed in order to extract DNA; the methods chosen can however directly
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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Genetic Transformation of Bacteria Abstract The transformation of bacteria was successfully carried out using a plasmid carrying a gene that codes for green fluorescent protein‚ which gives a signature green glow reminiscent of a jellyfish. This gene‚ however‚ is only active when the sugar arabinose is present. A gene coding for antibiotic resistance was also found within the plasmid and served as a means to verify that transformation had indeed taken place. The hypothesis was that the bacteria
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Anthony D. Sucgang BMLS-IA Electrophoresis Machine Gel Electrophoresis Apparatus(Aragose Gel) Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. In this lesson‚ we’ll review how agarose gel electrophoresis works and introduce the equipment necessary to perform an electrophoresis experiment. Separation
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Page 1 Analysis of transcriptional regulation of the arabinose operon promoter using expression from a green fluorescent protein encoding reporter gene + + KIRA FERNANDEZ ‚ CLAIRE MARKEY * ‚ JESSE PIERATTI + Department of Biological Sciences ‚ and Department of Nutrition and Dietetics*‚ Messiah College‚ Mechanicsburg‚ PA 17055 Page 2 ABSTRACT This study investigates gene regulation and how environmental arabinose and/or glucose can interact with geno
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instead of preparing one large dilution‚ if we take a concentrated sample of a particular compound and perform a series of dilutions with it‚ we can obtain various concentrations of the same compound. In the context of this experiment‚ for gel electrophoresis and absorbance spectrophotometry‚ we need to prepare a series of
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