3. Study your gel electrophoresis results: a) Which solution sample contained a single dye: S1‚ S2‚ or S3? How do you know? S3 contained a single dye because it only had one band. b) The molecular weights for the dyes are 452.38 atomic units (au) for orange G‚ 669.98 au for bromophenol blue‚ and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes? Base on the order of movement of the dyes through the gel (yellow moved the
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“compositase” to find the identity of the plant in the pot. This process uses electric currents to draw DNA out from the wells formed in the gel because the DNA is negatively charged and it would try to move through the gel to get away from the negative side of the electric current. This allows the scientists to see it’s behavior as it moves through the gel as the smaller proteins move more quickly than the larger ones. Since DNA is colorless the scientists stain the sample in order to visualize
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in a gel-electrophoresis box. Restriction endonucleases are critical tools in recombinant DNA methodology. Electrophoresis is the method of determining the size of fragments that are cut by restriction enzymes. These restriction enzymes always cut at their specific protein recognition sites. This is very useful in the sense that no two restriction enzymes codes for exactly the same recognition site‚ giving it a unique characteristic that is specific for a strand of DNA. Gel electrophoresis is a technique
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Plant Mini Kit-QIAGEN (Lot No: 142338933) according to manufacturer’s instructions. The quality and quantity of the extracted RNA was determined by a NanoDrop spectrophotometer (BioTek‚ EPOCH‚ serial 121004C‚ USA)‚ and confirmed by agarose gel electrophoresis. After synthesis of cDNA‚ PCR was conducted and expected results were shown. qRT-PCR was performed using BioRad system with the fluorescent dye SYBR®Green Master Mix 2X (Ampliqon‚ cat No.: A325402‚ Denmark) in accordance with the manufacturer’s
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last tube (negative control) contained PCR master mix with sterile water. A negative control was used to determine whether or not cross contamination had occurred. - Agarose Gel Electrophoresis: The gel electrophoresis was used in order to determine and visualize and size the plasmid DNA and the PCR product. The gel electrophoresis was prepared using
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students in a BIOL 3600 laboratory. Buccal cells were extracted from the BIOL 3600 students‚ later purified‚ and then cut with two restriction endonucleases‚ MseI and RsaI. The now cut DNA was mixed with a blue loading dye and run on a 1.0% agarose gel (with added TAE buffer and Sybr Safe) at 110 volts. The finished product had bands of diverse base pair lengths‚ differing from student to student. During the RFLP analysis
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Using Kanamycin Resistance Bacteria to find the Sources of Contamination of Three Chicken Farms Introduction: Kanamycin is a common antibacterial that interferes with bacterial growth‚ by inhibiting protein synthesis‚ and causing the mistranslation of mRNA. Kanamycin is commonly used in chicken feed to keep harmful bacteria from getting into the eggs and producing healthier chickens. Recently reports of severe gastroenteritis have been linked to eating raw or undercooked eggs. This has led to
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DNA gel electrophoresis. They are both used to separate proteins by charge‚ in that positively charged molecules move towards the he negative electrode; negatively charged molecules move towards the positive electrode. The only difference is SDS is used to denature proteins and coat them so that they all carry negative charges although DNA is already negatively charged. But‚ protein can have numerous net charge‚ so it is essential that they are coated with SDS before it moves through the gel. Laemmli
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an entire species history. METHODS: Isolation of Fish Protein using Lamelli Buffer Before starting anything we labeled 1.5 ml flip-top microtubes and screw top microtubes 1 through 8 for each fish sample that is being prepared for electrophoresis. Lamelli sample buffer is added to each flip-top microtube in 250 µl increments; this buffer is used to denature the fish protein. Our fish protein will come from eight different fish muscle samples that are cut in a cube that is approximately
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Basic Tools for DNA Manipulation and Molecular Genetic Analysis Enzymes Vectors Gel Electrophoresis PCR Southern blotting Gel Electrophoresis Gel is a complex net work of polymeric molecules Both DNA and RNA are negatively charged owing to phosphate backbone Nucleic acids migrate in a gel toward anode (+) at a rate that is inversely proportional to the log10 of the molecular weight Gel Electrophoresis Migration of nucleic acids depends on pore size (small molecules migrate faster than
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