3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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Relationship and biodiversity Introduction Botana curus is a valuable plant because it produces Curol‚ a compound used for treating certain kinds of cancer. Curol cannot be produced in the laboratory. Botana Curus grows very slowly and is on the endangered species list‚ so its ability to provide Curol in large quantities is limited. Species that are more closely related to Botana curus are more likely to produce the important substance Curol. Three similar plant species that are plentiful (X
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Who Killed Rockina? The Crime Late one night‚ the famous rock star‚ Rockina‚ returned to her luxurious apartment from an appearance at a Concert. As she entered her locked apartment‚ she noticed that everything in her apartment was a mess -- the drawers had been emptied out onto the floor; the cushions on the couch were ripped open; and the safe behind the picture on the wall had been opened. She then noticed that the lights were on in her bedroom. She stormed into the bedroom and surprised
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Identification of Mystery Plasmids Abstract Plasmids serve as important tools in genetics and biotechnology labs‚ where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size‚ antibiotic resistance‚ lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be
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cells. This is done using a Zymography Electrophoresis gel.
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into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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SDS–polyacrylamide gel electrophoresis‚ and differential scanning calorimeter (DSC) is to be followed. Process: It is indicated in the diagram on how we can be able to extract our collagen from Janitor fish skins and what tests are needed to determine the properties of the extracted Acid soluble collagen and Pepsin soluble collagen. To compare amino acid profiles similarities w/ a collagen we used Amino acid analysis by AOAC method. Then to determine peptide mapping we used SDS–polyacrylamide gel electrophoresis
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Assignment 2: Answer Sheet (LSM 1401 Semester 2 AY2011/12) Nucleic Acid Virtual Laboratories |Name | | |Matriculation number | | |A. DNA Extraction Virtual Lab [2 marks]
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sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through the gel and the sequencer reads the bases
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The Polymerase Chain Reaction and Determination of Alu Population Freqencies Porshia Gibbs April 8‚ 2010 Genetics Laboratory Abstract Cheek cells were extrapolated and used in PCR amplification and electrophoresis of the amplified samples to determine the presence or absence of the dimorphic Alu sequence in a class population. A bioinformatic allele server was then employed to calculate genotypic and allelic frequencies of the Alu element in the class population. The Hardy-Weinberg equation
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