Analysis of the RBM22 Protein Association with Gene Expression through Affinity Tagging Lucas Akin Stream Faculty: Scott Stevens Research Professor: Albert MacKrell University of Texas at Austin School of Biological Sciences Abstract Affinity tagging of proteins is an important method for biochemical analyses [4] and has proven effective in further expanding our knowledge of their specific functions. A group of interactive proteins expressed by bacteriophage ʎ known as exo‚ bet‚ and
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Beta Thalassemia Beta thalassemia is an inherited blood disorder characterized by the abnormal production of a blood protein called hemoglobin. This condition is caused by a mutation within the gene that is responsible for the healthy production of hemoglobin. In healthy people‚ hemoglobin carries oxygen to tissues and cells throughout the body. Patients with beta thalassemia do not have adequate levels of oxygen within the blood‚ which can cause anemia. There are two main types of beta thalassemia
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A2 BIOLOGY CORE PRACTICAL SUMMARY Name of practical Independent & dependent variables Observing patterns by Ecological sampling Random sampling Systemic sampling Other variables to be controlled Abiotic factors e.g. light‚ temperature‚ soil water‚ humidity‚ O2 concentration‚ pH‚ aspect‚ slope angle equipment Gridded Quadrat Tape measure Point quadrat Pitfall trap Sweep Net Pooter Tullgren funnel Baermann funnel Method and outcome Several methods. 1 random sampling = set up grid using
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Genes and Genetics 1. 2. each chromosome exists as two genetically identical chromatids attached to their centromere. Each chromosome appears as two chromatids attached to a centromere. In the first meiotic division chromosomes align in homologous pairs. Points of contact form between members of the same homologous pair. The points of contact or crossing over between members of a homologous pair are the chiasmata. 3. The homologous pairs move to the equator of the cell. Equal lengths of the
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After the linear equation was formed‚ the unknown sample concentration was determined using the standard curve equation. A Gel Electrophoresis was used to perform a qualitative analysis. The use of 5 microliters of the homogenate was heated to 80 degrees Celsius. Then the homogenate was transferred to a 2-microliter-protein gel sample buffer. Samples loaded on to the gel was run at 100 v and stained with comassie blue; observations were made next lab. (Clendening 2014) The amount of glucose
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Abstract This experiment investigated the kinetics of the enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen
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1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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will cut and the number and size of DNA pieces that will result. These pieces can then be separated and compared using a process called gel electrophoresis. The DNA moves from the negative end to the positive end. As the fragments move‚ their varying lengths propel them through an agarose gel at different speeds. Short strands move through the holes in the gel more quickly than long strands and will over time move farther away from the starting point. Staining the sorted groups of DNA makes them
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Systems Biology Systems biology involves the study of an organism as one single system. Instead of analysing all the individual components that make up a cell‚ the cell is instead viewed as an interacting network of genes‚ proteins and biochemical reactions and these are studied as a whole. In 20th century‚ molecular biology was focused upon. A ‘reductionist’ approach was followed‚ in which the individual components‚ such as the cell nucleus or sugar metabolism‚ were studied in isolation. However
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Antibiotic Production from the plant Lantana camara Project Summary The purpose of this experimental procedure is targeted to find a novel bacterium from the soil of Lantana camara that can be used in the dental field to kill or stop the growth of the Streptococcus mutans bacteria. The experimental procedure is to take place over a time frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil‚ find antibiotic
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