Chapter 12 Genomes Study Guide By: Divya Prakriya Concept 12.1 : There are powerful methods for sequencing genomes and analyzing gene products. • The goal of sequencing genomes is to identify mutations in DNA and relate them to phenotypes (ie. Understanding genetics) • Human Genome Project- 13 year project‚ used chemically modified nucleotides • Next generation DNA sequencing- uses miniaturization techniques 1st developed for electronics industry‚ as well as principles of
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numbers of repeats on both chromosomes‚ which is much higher than a 20% chance of being homozygous. MATERIALS AND METHODS In this experiment‚ we extracted DNA from cheek cells‚ loaded PCR products in a 1% agarose gel and took gel images. When loading our mixtures into the wells on the gel‚ we worked with positive and negative controls. The negative controls consisted of water and the positive controls consisted of purchased human DNA. The last two tubes consisted of buccal DNA. Please see lab manual
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Review Guide: General Questions/Skills: 1. Be able to draw a polypeptide given only the letter designations for the amino acids. 2. Given a list of 6 organisms (mentioned in class)‚ be able to draw the big tree‚ labeling three domains‚ the root‚ and relative branch lengths for the domain. Origin is on bacteria line in the middle. Archaea are generally shorter lines‚ bacteria are the intermediate lines and eukaryotes are the longest lines. Organisms: Are there going to be specific
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investigators use biometrics to determine who is and who is not the perpetrator of a crime. How crime investigators use biometrics to determine who is and who is not the perpetrator of a crime is they a RFLP analysis and DNA fragments are separated by gel electrophoresis. 6. Applying Information: Why should both individuals and society address ethical concerns? Explain Individuals and Society should address ethical issues because both sides raise good points and whenever scientists use Biotechnology
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pressure‚ depression in freezing point and elevation in boiling point. Electrophoresis The movement of colloidal particles under the influence of electric field is called Electrophoresis. When an electric field is applied across the colloidal solution‚ the colloidal particles migrate to oppositely charged electrode where they get neutralized. This phenomenon is known as electrophoresis. Electro Osmosis When electrophoresis of dispersed particle in a colloidal system is prevented by some suitable
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Abstract Population genetics is the study of how localized groups of individuals capable of interbreeding and creating fertile progeny change genetically over time. The Hardy-Weinberg equilibrium accounts for gene pools that do not change genetically over time. In this experiment‚ I intended to determine whether the sample population consisting of my fellow biology lab classmates would fall in the Hardy-Weinberg equilibrium with respect to the ALU insert from human chromosome 8. My hypothesis was
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Summary for Quiz Monday‚ May 30‚ 2005 12:13 AM Title Identification of Some Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict’s test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory Introduction • The most abundant elements in living material are: o Carbon
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Google Slides At 9:45 PM on October 5‚ 2005‚ Esteban Julio Roberto Montoya Dela Rosa Ramirez was found dead in an the elevator he operated at the Ritz Palace hotel. When the policeman was alerted by a teary hotel clerk at 9:55 PM‚ he rushed to the scene. He found Esteban Julio Roberto Montoya Dela Rosa Ramirez with a stab wound in his chest. Upon further inspection the investigative team found a large bruise on the back of the victim’s head. The hotel clerk was taken back to the police station
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series of labs‚ we were able to extract and isolate our mitochondrial DNA‚ nuclear DNA‚ and cellular DNA to then amplify a part of an intron in the tissue plasminogen activator gene (TPA) through Polymerase Chain Reaction (PCR) and run on an agarose gel to detect the presence or absence of a transposable element known as an Alu element. The main purpose of this experiment was to use our class as a population‚ to test whether or not we were in Hardy-Weinberg Equilibrium. The Hardy Weinberg Law states
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Main topics on midterm labs 1-9 lectures 1-9 Operon; bacterial promoter; Rbs; DNA polymerase vs RNA polymerase function (lec #1) - Transcription is carried by RNA polymerase that RNA polymerase recognizes to a specific sequence (the promoter) and start making mRNA next to that position. - Usually‚ typical bacterial promoter carries tow consensus sequences (the sequence that all the organism shares) TTGACA at position of -35. TATAAT at -10. - As mRNA is being transcribed
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