Conclusion In part A of this experiment‚ we transformed the bacteria into an antibiotic resistant form by inserting a plasmid into it. We used heat shock in order to make the bacteria capable to uptake a plasmid in the presence of calcium ions that help disrupt the cell membrane (heat shock is the combination of altering hot and cold). When they are capable of accepting plasmids‚ the bacteria are incubated with plasmids that carry the resistance to a particular antibiotic‚ in this case ampicilin
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Chapter 20: DNA Technology Biotechnology: Use of living organisms to perform tasks. * Wine & cheese * Selective breeding * Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand‚ but in the opposite direction * The exposed bases provide “sticky ends” * H-bond to compliment bases of segments cut with same restriction
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from the bioinformatics database‚ we can actually introduce the virus into the cell‚ comparing it with a non-infected cell. SDS-PAGE or 2-dimentional electrophoresis can be used to detect the differences between the two: targeting proteins will exist in the non-infected cell but will not exist in the infected cell. Two-dimensional gel electrophoresis will separate proteins on the basis of charge and mass. Finally‚ we can obtain the pure targeting protein‚ and final step is to identify the amino
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and added sterile beads to spread the bacterial cells all over the surfaces of the two agars and removed the beads and incubated the two agars. While the agars were incubating‚ we prepared our gel and loaded our respectful samples and ran the gel. After the gel finished running‚ we got a picture of our gel and recorded our observation for later
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Beach Channel High School Dr. David Morris‚ Principal The Living Environment Laboratory Study Guide The Living Regents Examination is at 9:00 a.m. on Friday January 26‚ 2007 Lab Activity 1 - Relationships and Biodiversity In this lab‚ students are introduced to classification and the importance of biodiversity. Organisms are according to similar characteristics. Some of these characteristics are physical (structural) and others are Biodiversity is the amount of the different organisms
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was introduced in different parts of Asia and had been an invasive species and a pest in different ecosystems ever since the introduction. In understanding this species of snails‚ samples were collected‚ DNA’s were extracted‚ undergone PCR and electrophoresis‚ and was sequenced and analyzed. The analysis was qualitative In the Philippines while quantitative in China. In the Philippines‚ the cytochrome oxidase subunit 1 (COI) genes was used and compared among the species collected and when it was sequenced
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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Abstract Aquilaria malaccensis is an agarwood tree that originate from a place called Malacca. Among other aquilaria species‚ aquilaria malaccenis has the best quality of the fragrant resin. Therefore‚ due this unidue characteristic‚ A.malaccesis has been listed in IUCN as an endangered tree. In this study‚ the aim was to record the morphology of the A.malaccensis and examine the genetic variation among the A.malaccensis. This is because although they are the same species‚ but some tree had shown
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* Restriction endonucleases- recognize specific sequence of DNA and break phosphate-sugar bond. * Liagase- rejoins phosphate-sugar bonds cuts by endonucleases. * Reverse transcript-makes a DNA copy of RNA. * Analysis of DNA * Gel electrophoresis- separates DNA fragments based on size. * Nucleic acid hybridization and phrobes- probes based pair with complementary sequence used to detect specific sequences. * DNA sequence- reading the sequence of nucleotides in a stretch of DNA
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reaction‚ or PCR. PCR produces millions of copies for each DNA segment of interest and thus permits very minute amounts of DNA to be examined. The resulting PCR products are then separated. The separation methods used today include slab gel and capillary electrophoresis. Fluorescence detection methods have greatly aided the sensitivity and ease of measuring PCR-amplified samples. The specific methods used for DNA typing are validated by individual laboratories to ensure that reliable results are obtained
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