MCB 3020 Exam TWO Study guide 1. List the laws of thermodynamic and describe their relevance in the chemical reactions 2. Define the standard reduction potential. Why aerobic grow generates the highest amount of energy (ATP). How this value plays a role in organization of electron transport system. Compare Eo of aerobic and anaerobic respiration 3. Describe the flow of electron in fermentation and respiration. What happened to the electron in each of the above processes 4
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protocol. In order to prevent from DNA contamination‚ bacterial suspension treated with DNA-free DNase set I (Qiagen‚ Germany). Then‚ density of RNA sample and its integrity were checked by Nanodrop spectrophotometer (Biochrom‚ England) and gel electrophoresis‚ respectively. According to manufacturer’s protocol‚ 100 ng of RNA sample was transcribed by using available random primer and RevertAid Reverse Transcriptase enzyme (RevertAid™ First Strand cDNA Synthesis Kit‚ Fermentas) and supplied inhibitor
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Homologous Chromosomes and Sister Chromatids (HC and SC): Clearly distinguish between HC and SC pairs. Your answer should include the following: a clear distinction between ‘chromatin’ and ‘chromosome’‚ and how this difference affects gene expression; simple line diagrams to illustrate the difference between non-replicated and replicated DNA inchromatin and chromosome forms; afull description of both HC and SC with a labeled diagram that includes gene/allele sequences for a heterozygote and ‘m’
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The Defective Kernel1 (DEK1) is a highly conserved membrane-anchored gene which is found in most of the land plant and this gene belongs to the calpain family that plays an important role in plant body organization. This study was done by taking Physcomitrella patens as a model plant because they possess DEK1 as the dominant phytocalpain. The study was mainly focused upon the comparision of steady state level of dek1 transcripts in wild type (WT)‚ dek1 non-expressing PpΔdek1 and dek1 overexpressing
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Jacob Pastva College Writing II Professor Wollenzier 25 September 2012 Back to Your Roots The origin of man has always been a question that the human race has tried to answer‚ but could never quite pinpoint. Homo Sapiens now know the exact start of man‚ due to advances in harvesting mitochondrial DNA. “Markers” are used to trace ancestry. These markers are found through DNA Sequencing and SNP testing. The general acceptance is that the human race stemmed from a woman referred to as “Mitochondrial
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Unit 5 Describe the two process by which hormones can cause certain genes to be turned on. (4) Any 2 from: - A hormone binds to a receptor in the cell surface membrane - Idea of the receptor causing (chemical) changes inside the cell - A (functional) {second messenger} activates an {enzyme/transcription factor/eq} Any 2 from: - Idea of a hormone entering the cell through the cell membrane - The hormone binds to a receptor - The {hormone-receptor complex/eq} acts as a transcription factor
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Phytochemical march allowed qualitative determining of the main groups of chemical constituents present in Licania arborea leaves. Abundant amount of amino acids‚ phenolic compounds‚ triterpenoids and tannins‚ moderated presence of flavonoids and leucoantocianidines‚ and the absence of nafto- and antroquinones‚ cardiotonics and alkaloids was observed. The high content of phenolic compounds happens to be interesting as there is a high probability polar extract from the leaves of this plant have antioxidant
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NO.102/1 Marker Code Student Personal Identification Number (SPIN) South Pacific Form Seven Certificate BIOLOGY 2009 QUESTION and ANSWER BOOKLET Write your Student Personal Identification Number (SPIN) on the top right hand corner of this page and on the fold-out flap on the last page. Answer ALL QUESTIONS. Write your answers in the appropriate spaces provided in this booklet. If you need more space for answers‚ ask the Supervisor for extra paper. Write your SPIN on all extra sheets used and
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Current Research‚ Technology and Education Topics in Applied Microbiology and Microbial Biotechnology A. Méndez-Vilas (Ed.) _______________________________________________________________________________________ Comparison of methods for the extraction of bacterial DNA from human faecal samples for analysis by real-time PCR E.A Nelson1‚ 2‚ E.A Palombo1‚ and S.R Knowles2 1 Environment and Biotechnology Centre‚ Faculty of Life and Social Sciences‚ Swinburne University of Technology‚ John Street
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* State the biotechnology is the industrial use of living organisms to produce food‚ drugs of other products * Explain why microorganisms are used in biotechnological processes * Describe the standard growth curve in closed culture * Compare and contrast the processes of batch and continuous culture * Describe the difference between primary and secondary metabolites * Describe the importance of manipulating the growing conditions in a fermentation vessel in order to maximise the
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