was designed to use western blot analysis to detect proteins from Non-fat powdered milk. The experiment had to be run two times before the expected outcome was reached. However‚ after the second attempt‚ it was successful. The gel ran well‚ and the proteins from the gel were successfully transferred to the membrane provided in the kit. Introduction The name western blot was given to the technique by W. Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by
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BSC 2010C.LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the
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Lab Report Electrophoresis: Using Electricity to Separate Molecules Answer the following questions about the results of this activity. Record your answers in the boxes. Send your completed lab report to your instructor. Don’t forget to save your lab report to your computer! Activity 1 – Calibration Record your data from Activity 1 in the boxes below. Enter the data (number of bands‚ description of the band patterns) you collected for the protein ladder in the appropriate columns
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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we confirm the high-level expression of HIV-1 p24 antigen in transgenic tobacco plants through plastid transformation. PCR analysis confirmed the presence of the HIV-1 p24 sequence within the chloroplast genome of transgenic lines. SDS-PAGE gel electrophoresis of protein extracts from transgenic plants identified plant-expressed HIV-1 p24 protein. Quantification of the recombinant protein HIV-1 p24 using Aligent 2100 Bioanalyzer estimated yields of about 13 mg per g of soluble leaf protein. Our results
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2.6 Kinetic studies of prepared complexes The integral method of Coats–Redfern equation[19‚21‚27‚38] was used for determining the kinetic parameters of the decompositions process for the investigated metal complexes according to following equation: log[log(w_∞/(w_∞-w))⁄T^2 ]〖=log[AR/〖∅E〗^* (1-2RT⁄E^≠ )]〗-E^≠/2.303R 1/T (4) Where w_∞ is the mass loss at the accomplishment of the decomposition reaction‚ w is the mass loss at temperature T‚ ∅ is the rate of heating and R is
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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encoded fusion proteins was assessed by SDS-Page. Analyzing the gel under fluorescent conditions reveals protein bands which have the HaloTag® ligand attached. The PageRuler Plus prestained Protein ladder possesses two fluorescent bands‚ at 25 and 70 kDa respectively. HaloTag® Standard Protein with a size of 60 kDa was also analyzed and helps as an additional size reference. The HaloTag® features a size of 34 kDa alone. Agarose gel of PCR products after amplification of Campylobacter jejuni genes
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Enzymes and Electrophoresis Bhumik Patel Phillips 1/16/11 Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough‚ while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation of DNA. Gel Electrophoresis
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typically the most laborious aspect of protein purification. The most abundant protein found in milk is caseins and it can be removed by heat and low pH. The most general method to monitor the purification process is by running a SDS-polyacrylamide gel electrophoresis of the different steps. This method only gives a rough measure of the amounts of different proteins in the mixture‚ and it is not able to distinguish between proteins with similar apparent molecular weight. the purest‚ isolated form of bovine
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