myself or your TA. Plagiarism will result in an automatic zero. 1. In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide gels. List all of the ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? The percent acrylamide refers to the size of the pores as percent acrylamide increases the size of the pores decreases
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The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
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* To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results 1 2 3 4 5
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lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus will be connected
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Colon Cancer Scenario Introduction: Gel electrophoresis is an important molecular biology tool: it enables us to study DNA. It can be used to determine the sequence of nitrogen bases‚ the size of an insertion or deletion‚ or the presence of a point mutation; it can also be used to distinguish between variable sized alleles at a single locus and to assess the quality and quantity of DNA present in a sample. Gel electrophoresis is a method of separating chemical compounds and molecules by their size
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DNA Isolation and Visualisation The laboratory work today consists of three stages. 1. Separation and visualisation of DNA on agarose gel 2. DNA extraction 3. Virtual electrophoresis Part 1 involves casting a gel‚ which then requires approximately 30 minutes to set‚ followed by 1 hour to run. Consequently‚ you might want to cast the gel and let it set whilst you undertake Part 2 - your DNA extraction. Make
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Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction enxymes and separated using gel electrophoresis‚ then
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prevalence of the Borrelia‚ Rickettsia and Ehrlichia species (the causes of the diseases mentioned above) in the tick A. inornatum‚ the Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis procedures are applied. The PCR method is used to amplify the DNA samples of the tick studied‚ while the Agarose Gel Electrophoresis method is run in order to identify whether the species of bacteria of our interest are present in the DNA samples or not. The results obtained‚ so far‚ show 23.5% positive for
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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4 Cardiovascular disorders CASE STUDY 19 Annie’s heartache Learning outcomes On completion of this case study‚ you will be able to: • describe the physiological characteristics of the coronary circulation; • outline the autonomic control of the heart and the mechanisms which control coronary blood flow; • review the causes of and possible treatments for angina; • describe the mechanisms of action of the following anti-anginal drugs: nitrates‚ beta-adrenoceptor antagonists and calcium
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