Virology –Midterm vA‚ 22 April 2013: 1. Which of the following genomic nucleic acids are only found in viruses? a. dsDNA b. dsRNA c. ssDNA d. ssRNA e. B‚ C and D 2. About what percent of the human genome is indisputably viral? a. 1 b. 2 c. 5 d. 10 e. 50 3. Viruses were first discovered (and named as such) because they : a. could not be grown b. were very small c. were alive d. ate bacteria e. C and D 4. Phage therapy is to : a. Use a virus to kill a virus b. Use viruses to kill cancer cells c. Use
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2.4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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The results of the experiment supported that invertase is most active at a pH level of 5‚ which supports the hypothesis that lower acidic pH values similar to the ones found in the stomach‚ will have the largest increase in invertase activity. A semilinear decrease in rate was found as the pH level was lowered below 5‚ and a substantial decrease found in any environments with a pH higher than 5. The stomach average pH level‚ being between 1.5-3.5‚ directly correlates to the most efficient pH levels
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How does the process of gel electrophoresis separate DNA fragments? It uses an electric current to separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has an
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experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is placed in a chamber with positively and negatively
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DIGE-based PTMs Analysis of protein post-translational modifications using DIGE-based proteomics Robert M. DeKroon‚ Jennifer B. Robinette‚ Cristina Osorio‚ Sun Yong Jeong‚ Eric Hamlett‚ Mihaela Mocanu and Oscar Alzate Summary Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples‚ and this information is valuable in making inferences about relative protein activity. However‚ a protein‟s activity
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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Nick Sarris‚ April 3‚ 2013‚ D-Bell Biology Virtual Electrophoresis Lab – Genetic Science Learning Center Use the link to complete the following lab. Submit through edline when you are finished http://learn.genetics.utah.edu/units/biotech/gel/ Title‚ name‚ date and bell (8 pts) Place your answer below the question and skip between questions (2 pts) Each question is worth 3 points 1. Why can’t DNA be sorted physically‚ using a microscope?- They are so tiny that they are unable to be
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