the break allows DNA to rotate around the phosphodiester backbone which causes supercoils to be released. Supercoiled plasmid is more compact and sustains less resistance compared to open circular plasmid and therefore travels quicker in an agarose gel. Linear conformation is produced when a smaller fraction of the DNA sustains double-stranded breaks and it has less resistance than open-circular DNA‚ but more resistance than supercoiled DNA (Varsani
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setup and the water agar-gel setup. In the glass tube setup‚ two cotton balls were soaked in the solutions of hydrochloric acid (HCl) and ammonium hydroxide (NH4OH) and were simultaneously placed on both ends of the tubing.NH4OH had a lighter molecular weight of 35 g/mole which diffused at a faster rate of 24.8 cm and formed a white smoke near the HCl end that had the molecular weight of 36 g/mole. The water agar-gel setup was made up of a petri dish containing the gel with three wells. Drops
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3: The results of Experiment 3; Protein Precipitation Precipitant Observation TCA White fluffy precipitate in large mass‚ the fluffy mass goes from the bottom to the top of the tube. The solution was thin and loose. Ammonium sulphate White gel-like precipitate‚ the solution was thick. HCl White precipitate accumulated at the top and also the bottom of the solution. The solution was thin. NaOH No precipitate seen‚ the solution was cream in color. Copper sulphate Light blue coagulated
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variation of fill weight. (with a liquid‚cV< 1% possible) . 2. Patient safety compliance/consumer preference Softgel capsule shel soft/flexible; popular dosage form‚ due to ; Ease of swallowing Absence of taste Convenience (portable‚ robust) Soft gels capsules can be formulated to be taken in different ways; Chewable or lozenges Twist-offs (with a tag allows access to contents) 3. Increased Bioavailability Dissolution of drug from solid state (i.e formulated as an ordinary tab/cap) can be rate
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Tetraphenylporphyrin Abstract: In this green chemistry experiment of 5‚10‚15‚20-tetraphenylporphyrin was synthesized from benzaldehyde and pyrrole. using microwave irradiation to heat the reactants.‚ The liquid reactants are absorbed on a solid support‚ silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin
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Examining the RNA Interference Mechanism in the dpy-13 Gene in C. Elegans Through Feeding Mehdi Misto Lab: Monday 1:00 – 4:50 PM 11 December 2012 Introduction: RNA interference‚ or RNAi‚ is a biological process in which RNA molecules reduce the gene expression of an organism. This is done typically by causing the destruction of specific mRNA molecules. RNAs are direct products of genes‚ these small RNAs can bind to other mRNA molecules to either increase or decrease their activity like in the example
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ABSTRACT The microevolution of Alu element TPA-25 was tested in the experiment through the examination‚ observation‚ and analysis of population alleles distribution under the Hardy-Weinberg Theory of Genetic Equilibrium. Alu elements affect the genome by causing insertion mutations‚ recombination between elements‚ gene conversion‚ and alterations in gene expression. In the lab PCR was used to amplify a short piece of DNA from human genome which allowed us to look for a DNA sequence called an Alu
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Genetic Transformation of Bacteria Abstract The transformation of bacteria was successfully carried out using a plasmid carrying a gene that codes for green fluorescent protein‚ which gives a signature green glow reminiscent of a jellyfish. This gene‚ however‚ is only active when the sugar arabinose is present. A gene coding for antibiotic resistance was also found within the plasmid and served as a means to verify that transformation had indeed taken place. The hypothesis was that the bacteria
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start aloe-vera project by producing & Providing Cosmetics products for Body Care‚ Face Care & Hair Care. Body Care : a) Body Lotion‚ b) Body Massage‚ c) Body Wash Face Care : a) Face Wash‚ b) Face Gel c) Face Cream Hair Care : a) Hair Removal Cream‚ b) Hair Gel‚ c) Hair Oil Business Size : We prepare our business plan for Two years‚ which is start by producing above Nine products. First year we expect the business like‚ sales will be tk. 2.5 Core‚ Capture market share 12%
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The wild type and the mutant type strains of Halobacteria were used in this experiment to examine the phenotypic differences against each strain’s genotypes. The mutant strain (KBT-1) did not possess gas vesicles‚ which decreased its ability to float to the surface. The lack of gas vesicles in the mutant strain made the colonies a red color. The wild type strain (NRC-1) had gas vesicles and appeared a pink pigment color. Growth on the agar allowed one to examine the specific colonies. Inoculation
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