nanodrop-spectrophotometer to determine concentration and purity of our product. Gel electrophoresis: We performed agarose gel electrophoresis to separate DNA fragments by size. Since DNA has a negative charge‚ it migrates towards the positively charged site presence of an electric current. Agarose solution is prepared by agarose powder and TAE buffer. Ethidium bromide (EtBr) which is kind of mutagen is added for track DNA on a gel. EtBr binds DNA and makes it visible under the UV light. Then‚ the electric
Premium DNA Bacteria Gene
Chapter I PRODUCT SPECIFICATION I. Introduction Mango (Magnifera indicia) is a major tropical fruit that is widely consumed in the society. It is considered the dominant tropical fruit variety in the world (Sarris 2003) with total production worldwide of about 25 million metric tonnes a year. It is a succulent‚ delicious fruit with a high nutritional content that is commonly consumed when ripe. Mango is a seasonal fruit that is native to Southeast Asia. As a result of its sweet taste and the large
Premium Mango Kilogram Acid
2.3 Gel Electrophoreses After the PCR program completed‚ 2.7μL of 10X loading dye was transferred into each PCR tube. An electrophoresis gel chamber was completely immersed by 1X Tae buffer. Then‚ 10μL of DNA ladder was loaded in to the middle of the gel‚ and 20μL of each PCR sample were loaded into distinguish location in the gel. After all lanes are loaded‚ electrophoresis was turned on at a constant rate of 110-115V for one hour. 2.4 Gel imaging and analysis After the gel electrophoresis
Premium Genetics DNA Gene
EMBELLISHMENT ACCESSORIES WITH SPECIAL TREATMENTS AND MATERIALS DEREK CHUN KIT‚ CHAN BA(Hons) Scheme in Fashion and Textiles (Fashion Design Specialism) INSTITUTE OF TEXILES & CLOTHING THE HONG KONG POLYTECHNIC UNIVERSITY EMBELLISHMENT ACCESSORIES WITH SPECIAL TREATMENTS AND MATERIALS A Thesis Submitted In Partial Fulfillment of Requirements for the Degree of Bachelor of Arts (Honours) In Fashion and Textile (Fashion Design Specialism) Under the supervision of Dr. Jeanne Tan By Derek
Premium Plastic
1: Plasmid Isolation Gel Electrophoresis. This figure represents result from several groups doing plasmid isolation. All the data are collected in Bio 22 class on February 24‚ 2012. DNA obtained from this experiment comes from a plasmid DNA that were isolated and put into a buffer solution before running it through a gel. The result of our particular experiment is in lane 2. The sample in lane 2 is a control group which contains RNAse instead of DI water. This particular gel is 0.8% agarose. Figure
Premium DNA Molecular biology Genetics
osmosis has been seen and tested through experiment. In part A of the lab‚ diffusion was demonstrated with two solids and an agar gel petri dish. One crystal of potassium permanganate and one crystal of methylene blue were placed on either side of an agar gel petri dish. The purpose of this experiment was to determine which of the crystals would diffuse across the gel more. So the question is‚ which solid would have a higher rate of diffusion through the agar‚ methylene blue or potassium permanganate
Premium Chemistry Enzyme Concentration
Objective The objective of the project of redesigning the bicycle saddle is to lessen the uncomfortable sitting on the bicycle saddle‚ giving cyclist the maximum pleasure of riding of the bicycle. This is because the current bicycle saddle causes pain to the soft perineal tissue‚ the major part of the human’s tissue that has direct contact to the saddle. The pain that comes from the saddle is because all the pressure from the cyclists’ weight is directed onto the soft perineal tissue. Therefore
Premium Bicycle Cycling Human anatomy
Should all people convicted of a crime have their DNA fingerprints stored on a database?\ A DNA fingerprint is the same for every cell‚ organ and tissue in an organism. DNA fingerprinting has many uses‚ some of which include providing the evidence needed to solve criminal investigations‚ determining genetic relationships and solving paternity disputes. DNA fingerprinting has many benefits in the use of criminal investigations as it can provide the evidence to solve crimes and current mysteries
Premium DNA
discharge the entire volume of solution Press the tip discarder to remove it Remember to change tips between each solution PEDIGREE Carlos (ff) Maria (Ff) Eric (ff) Anna (Ff) Juanita (Ff) Jason (Ff) Erin (FF) GEL WITH PATIENT NAMES Lane #1: DNA Standard Markers Lane #2: Normal DNA Sample Lane #3: FH control (FF) Lane #4: Patient #1 (Carlos-Anna’s Father) Lane #5: Patient #3 (Anna
Premium DNA Molecular biology
market has been largely by the Gel Segment. Presently ‚a large chunk of the Market is still held by Colgate. The major players in the toothpaste Industry being Colgate Palmolive and Hindustan Lever Limited and several minor players like Balsara hygiene‚ Dabur etc. Presently Colgate Dental Cream holds 52% of market share. HLL’s Close up lies far behind with 23% of the existing market share. The third player in the market place in terms of market share is Colgate Gel with 10.5% of the market share
Premium Toothpaste Oral hygiene Trademark