Electrophoresis is commonly used to separate proteins using an applied electrical field. SDS-PAGE separates proteins by molecular weight‚ using sodium dodecyl sulfate (SDS; also known as lauryl sulfate) to denature them and a discontinuous polyacrylamide gel as a sieving matrix. The net charges of folded proteins are not dependent upon molecular weight. Rather‚ charge is determined by the sum of the individual amino acids’ R-group charges together with the molecular radius of the tertiary structure. Therefore
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POLYMER SYNTHESIS AND PROPERTIES AIM: The aim of the experiment was to prepare three different polymers‚ namely nylon 66‚ Agar Gel and slime‚ being able to differentiate between the configuration and analysis among the three structures‚ noting the physical characteristics of each polymer and the chemical reactions that occur during the formation of the polymer. Pre- lab questions 1. Is Nylon 66 a step or chain – growth polymer? Define both types of polymerization in your answer. Nylon 66
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the last tube (negative control) contained PCR master mix with sterile water. A negative control was used to determine whether or not cross contamination had occurred. - Agarose Gel Electrophoresis: The gel electrophoresis was used in order to determine and visualize and size the plasmid DNA and the PCR product. The gel electrophoresis was prepared using
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Figure 2 also indicated the success of the PCR amplification since we were able to construct the gel map found in Table 1. In Table 1‚ lanes 1‚ 3‚ 6‚ 9‚12 and 14 were expected not to show any DNA since they were not loaded. We used different primers for the positive and negative controls for the sole of validity and accuracy of the 100bp ladder when
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Introduction The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast‚ EAY 235 and EAY 431‚ which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion‚ which caused EAY 431 to be a deficient
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L. PETERSON/AP BIOLOGY The diagram below shows a segment of DNA with a total length of 4‚900 base pairs. The arrows indicate reaction sites for two restriction enzymes (enzyme X and enzyme Y). (A) Explain how the principles of gel electrophoresis allow for the separation of DNA fragments. (B) Describe the results you would expect from electrophoretic separation of fragments from the following treatments of the DNA segment above. Assume that the digestion occurred under appropriate
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Potassium Dichromate‚ and Methylene Blue.1 Arantxa Alex Carpio Group 1 Sec. X – 4L March 24‚ 2015 ABSTRACT The effect of molecular weight and time on the rate of diffusion was determined using the agar-water gel test. A petri dish of agar-water gel with three wells was prepared and a prepared solution of each substance was dropped on each well; one with potassium permanganate (KMnO4)‚ the other with potassium dichromate (K2Cr2O7)‚ and the last one with methylene blue. The average
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09/05/2014 Group details: Odd week‚ Friday lab Table of Contents - SUMMARY/ABSTRACT This laboratory experiment has as objective to introduce to the rheological properties of drilling fluid‚ including plastic viscosity‚ apparent viscosity‚ Gel strength(GS)‚ exponent of ffow behaviour of power fluid (n)‚ consistency index of power law fluid(k) and yield point (YP). It is also an objective of this experiment to measure the rheological properties using an equipment called FANN viscometer and
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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