solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary phase. In the second week‚ the students will separate a methylene blue/sodium fluorescein mixture by column chromatography with silica gel as the stationary phase. Part I: Thin Layer Chromatography (TLC) 1. Obtain 2 silica-gel-coated TLC sheets and 1 alumina coated TLC sheet. Measure up 1 cm from the bottom of the TLC strip. Taking care not
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Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
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separate the charges using an electric field. This is done using the gel electrophoresis apparatus where the sample of substance is dispensed into a well in the gel material. The gel is then placed in an electrophoresis chamber‚ which is connected to electricity. When the current is applied‚ the larger molecules move more slowly through the gel and the smaller molecules move faster. The different sized molecules form distinct bands on the gel which will be analysed by scientists. X-ray During the latter
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METHODS The method used in this lab to map the proteins was the method of Polyacrylamide gel electrophoresis. This method can be used to separate the proteins present in the fish muscle and separates them on size. Due to the fact that they are separated by size‚ the proteins can be compared because similar proteins with stop at the same spot in the gel. So measuring the bands that show up on the gel you can determine if different fish species have similar proteins. The first thing that is
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sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8.8 and stacking gel‚ prepared using 0.0625 M Tris-HCl at pH 6.8. Results showed multiple bands located on the upper half of the gel‚ which suggested heterogeneity
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Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates‚ but others because of their special needs‚ or because they need special additives or a special thickness‚ coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed‚ then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that holds
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as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is placed in a chamber with positively and negatively charge cords on each side of the chamber in order to separate different DNA fragments. The distance that DNA fragment travels depend on its size and
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Enhancing Mousse‚ Suave Maximum Hold Gel‚ Sunsilk Captivating Curls Scrunching Mousse and L’Oreal Tightly Wound Curl Boosting Spray Gel all “work”‚ are affordable. You get your money’s worth and you are not left with a sticky residue‚ but the fact that no build up is left after using Sunsilk Captivating Curls Scrunching Mousse is what makes it the best choice to use on curly hair. TREsemme Flawless Curls European Curl Enhancing Mousse‚ Suave Maximum Hold Gel‚ Sunsilk Captivating Curls Scrunching
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proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a solid membrane (PVDF or Nitrocellulose) for WB analysis. For this procedure‚ an electric current is applied to the gel so that the separated proteins transfer through the gel onto the membrane. To detect the antigen
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tool in order to compare the mobility on agarose gels with dna markers of known lengths. Dna is a polymer that is negatively charged due to the sugar phosphates. When dna is on an electric field such as the electrophoresis gel the different lengths of dna migrate at different rates when they move through the porous gel. The ends of the gel are marked with a negative and a positive charge this forces the negative charged dna to move through the gel which can separate the dna by their lengths. In being
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