Dr.Tawfeq al.Qaimare Rifqa Shawer Lab report 5 21-3-2013 Adsorption Chromatography Introduction: The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube‚ it’s a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column‚ giving this region a nearly dark blue color. However‚ when
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The chemistry of hairspray What is hairspray: introduction Hair spray (or hair lacquer) is a common household aqueous solution that is used to keep hair stiff or in a certain style. Weaker than hair gel‚ hair wax‚ or glue‚ it is sprayed to hold styles for a short period of time. Using a pump or aerosol spray nozzle it sprays evenly over the hair. Hairspray was first developed and manufactured in 1948 by Chase Products Company‚ based in Broadview‚ Illinois.Its active ingredient is a suitable
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to separate mixtures into their compounds. It’s based on a mobile phase and a stationary phase. The mobile phase moves up the thin layer paper by taking the compounds with it‚ till it’s absorbed and stops moving. [1] The paper chromatography (silca gel) is a very uniform absorbent paper. Different components travel at different rates. Depending on the affinity each component has‚ it will move faster or slower up the TLC. To visualize the location of the different spots‚ an ultraviolet lam is necessary
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iIsolation of Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately
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Chapter I. The Problem and it’s Background Introduction Aloe Vera is a species of succulent plant that probably originated in northern Africa. The species is frequently cited as being used in herbal medicine since the beginning of the first century AD. Extracts from A. Vera are widely used in the cosmetics and alternative medicine industries‚ being marketed as variously having rejuvenating‚ healing or soothing properties. Aloe Vera is one of the only known natural vegetarian sources of Vitamin
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-Tique Corporation must decide if and how the company will introduce an aerosol can package for its Soft and Silky Shaving Gel‚ including a determination of product size (5.5 or 10 oz.) and location (personal care or toiletries). Furthermore‚ the manager needs to decide if a test market is necessary to evaluate the new packaging. Strategic Issues Product Soft and Silky Shaving Gel (SSSG)‚ a women’s shaving product that contains moisturizers‚ is currently packaged in a 5.5 oz. plastic tube and is located
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The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
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Colon Cancer Scenario Introduction: Gel electrophoresis is an important molecular biology tool: it enables us to study DNA. It can be used to determine the sequence of nitrogen bases‚ the size of an insertion or deletion‚ or the presence of a point mutation; it can also be used to distinguish between variable sized alleles at a single locus and to assess the quality and quantity of DNA present in a sample. Gel electrophoresis is a method of separating chemical compounds and molecules by their size
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DNA Isolation and Visualisation The laboratory work today consists of three stages. 1. Separation and visualisation of DNA on agarose gel 2. DNA extraction 3. Virtual electrophoresis Part 1 involves casting a gel‚ which then requires approximately 30 minutes to set‚ followed by 1 hour to run. Consequently‚ you might want to cast the gel and let it set whilst you undertake Part 2 - your DNA extraction. Make
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Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results 1 2 3 4 5
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